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PE anti-human CD27 Antibody

Pricing & Availability
Clone
M-T271 (See other available formats)
Regulatory Status
RUO
Workshop
V 5T CD27.03
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a.M-T271_PE_CD27_Antibody_FC_021113
Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
  • a.M-T271_PE_CD27_Antibody_FC_021113
    Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
  • b.M-T271_PE_CD27_Antibody__050222
    Human peripheral blood was stained with CD27 (clone M-T271) PE (solid line) or mouse IgG1, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
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356405 25 tests 62€
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356406 100 tests 162€
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T cells from a T-ALL patient.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma S, et al. 2011. J. Virol. 85:165. (IHC)
  2. Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
  3. Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  3. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  4. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  5. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  6. Kaku Y, et al. 2021. Cell Reports. 36(2):109385. PubMed
  7. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  8. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  9. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  10. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  11. Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
  12. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  13. Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
  14. Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
  15. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  16. Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed
RRID
AB_2561824 (BioLegend Cat. No. 356405)
AB_2561825 (BioLegend Cat. No. 356406)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Interaction
CD27 binds to CD70
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/13/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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