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PE anti-human CD177 Antibody

Pricing & Availability
Clone
MEM-166 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MEM-166_PE_CD177_Antibody_FC_091914
Human peripheral blood granulocytes were stained with CD177 (clone MEM-166) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • MEM-166_PE_CD177_Antibody_FC_091914
    Human peripheral blood granulocytes were stained with CD177 (clone MEM-166) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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315805 25 tests 104€
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315806 100 tests 240€
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Description

CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human granulocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.

Application References

(PubMed link indicates BioLegend citation)
  1. Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press.
  2. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed
  3. Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed
  4. von Vietinghoff S, et al. 2007. Blood 109:4487. (IF)
  5. Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)
RRID
AB_2564280 (BioLegend Cat. No. 315805)
AB_2564281 (BioLegend Cat. No. 315806)

Antigen Details

Structure
uPAR family, GPI-linked cell surface glycoprotein, 60 kD
Distribution

Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)

Function
Antigen involved in neutrophil allo- and autoimmunity, function unknown
Modification
Glycosylated
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002)
2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301.
3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108.
4. Temerinac S, et al. 2000. Blood 95:2569.

Gene ID
57126 View all products for this Gene ID
UniProt
View information about CD177 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09/24/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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