Pacific Blue™ anti-human CD66b Antibody

Pricing & Availability
G10F5 (See other available formats)
Regulatory Status
Other Names
Mouse IgM, κ
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Product Citations
Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
  • G10F5_PB_CD66b_Antibody_FC_032014
    Human peripheral blood granulocytes were stained with CD66b (clone G10F5) Pacific Blue™ (filled histogram) or mouse IgM, κ Pacific Blue™ isotype control (open histogram).
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305111 25 tests 109€
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305112 100 tests 268€
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CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  5. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
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  9. Bartsch YC, et al. 2022. Sci Transl Med. 14:eabn9237. PubMed
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  14. Adams LE, et al. 2023. Cell Rep. 42:112326. PubMed
  15. Bartsch YC, et al. 2023. iScience. 26:106582. PubMed
  16. Kaplonek P, et al. 2023. Nat Immunol. . PubMed
  17. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  18. Gorman MJ, et al. 2021. Cell Rep Med. 2:100405. PubMed
  19. Jundi B, et al. 2021. JCI Insight. 6:e148866. PubMed
  20. Boudreau CM, et al. 2020. J Clin Invest. 130:662. PubMed
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  26. Chen RE, et al. 2021. Med (N Y). 2:1327. PubMed
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  28. Gunn BM et al. 2018. Cell host & microbe. 24(2):221-233 . PubMed
  29. Offersen R, et al. 2020. Cell Rep. 33:108502. PubMed
  30. Cadena AM, et al. 2021. Nat Commun. 12:1474. PubMed
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  33. Dolatshahi S, et al. 2022. Sci Rep. 12:14937. PubMed
  34. Lu LL et al. 2016. Cell. 167(2):433-443 . PubMed
  35. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
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  38. Zelter T, et al. 2022. EMBO Rep. 23:e53641. PubMed
  39. Pullen KM, et al. 2021. Cell Rep. 37:109959. PubMed
  40. Bartsch YC, et al. 2021. Nat Commun. 12:1018. PubMed
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AB_2563293 (BioLegend Cat. No. 305111)
AB_2563294 (BioLegend Cat. No. 305112)

Antigen Details

Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD


Cell adhesion, neutrophil activation
Cell Type
Granulocytes, Neutrophils
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
View information about CD66b on

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Go To Top Version: 3    Revision Date: 01/05/2017

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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