- HCD54 (See other available formats)
- Regulatory Status
- Other Names
- ICAM-1, Ly-47
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD54 is a 85-110 kD type I transmembrane protein also known as ICAM-1. It is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The expression of ICAM-1 on the cell surface is potently upregulated by activation; a soluble form of ICAM-1 can be released from the cell surface. CD54 plays a role in cellular adhesion and is involved in inflammation and leukocyte extravasation. CD54 has also been shown to be the major cellular receptor for rhinovirus. ICAM-1 binds to CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) as well as hyaluronan and fibrinogen.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of lymphoctes interaction1. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 322721 & 322722).
(PubMed link indicates BioLegend citation)
- Evans HG, et al. 2009. Proc. Natl. Acad. Sci. USA. 106:6232. (Block) PubMed
- Product Citations
AB_893386 (BioLegend Cat. No. 322715)
AB_893384 (BioLegend Cat. No. 322716)
- Type I membrane protein, Ig superfamily, 85-110 kD
Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells. Cell surface expression upregulated upon activation; soluble ICAM-1 released from surface.
- Cellular adhesion, involved in inflammation and immune reactions. CD54 expressed on endothelial cells in areas of inflammation plays a role in leukocyte extravasation. Major cellular receptor for rhinovirus.
- CD11a/CD18 (LFA-1), CD11b/CD18 (Mac-1), CD11c/CD18 (p150, 95) and has also been reported to bind hyaluronan and fibrinogen
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Voraberger G, et al.. 1991 J. Immunol. 147:2777.
2. Staunton DE, et al.. 1988. Cell 52:925.
3. Greve JM, et al.. 1989. Cell 56:839.
- Gene ID
- 3383 View all products for this Gene ID
- View information about CD54 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD54 Reagents Request Custom Conjugation
|Purified anti-human CD54||HCD54||FC,ICC,Block|
|Biotin anti-human CD54||HCD54||FC|
|PE anti-human CD54||HCD54||FC|
|Alexa Fluor® 488 anti-human CD54||HCD54||FC|
|APC anti-human CD54||HCD54||FC|
|Pacific Blue™ anti-human CD54||HCD54||FC|
|Alexa Fluor® 647 anti-human CD54||HCD54||FC|
|FITC anti-human CD54||HCD54||FC|
|Ultra-LEAF™ Purified anti-human CD54||HCD54||FC,ICC,Block|
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