ELISA MAX™ Standard Set Mouse IL-10

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  • IL-10_Mouse_ELISAMax_081712
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431411 5 plates 212€
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Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation.

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-10 Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
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  2. Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
  3. Kubo T, et al. 2009. J. Exp Med. 206:1971. PubMed
  4. Asai A, et al. 2012. Immunobiology. 217:711. PubMed
  5. Baca Jones C, et al. 2014. PLoS One. 9:90855. PubMed
Product Citations
  1. Asai A, et al. 2012. Immunobiology. 217:711. PubMed
  2. Santos I, et al. 2020. Proc Natl Acad Sci U S A. 117:12281. PubMed
  3. Wang Y, et al. 2021. Andrologia. 53:e14239. PubMed
  4. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  5. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  6. Singh I, et al. 2018. Immunobiology. 223:549. PubMed
  7. Gomes MT, et al. 2022. J Biol Chem. 298:102034. PubMed
  8. Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
  9. Lin YZ, et al. 2022. Comput Struct Biotechnol J. 20:241. PubMed
  10. Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
  11. Croasdell A, et al. 2016. J Immunol. 196: 2742 - 2752. PubMed
  12. Hsieh PS, et al. 2021. Exp Ther Med. 188:21. PubMed
  13. Colaço HG, et al. 2021. Immunity. 54:53:00. PubMed
  14. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  15. Stravinskas Durigon T, et al. 2018. J Innate Immun. 10:279. PubMed
  16. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  17. Singh N, et al. 2016. J Immunol. 196: 1108 - 1122. PubMed
  18. Kumar RK, et al. 2021. Vascul Pharmacol. 138:106838. PubMed
  19. Egli N, et al. 2015. PLoS One. 10: 0142741. PubMed
  20. Lajqi T, et al. 2021. Int J Mol Sci. 22:. PubMed
  21. Fenoy I, et al. 2015. Exp Parasitol. 154: 47-50. PubMed
  22. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  23. Hu Y, et al. 2017. Biochim Biophys Acta. 10.1016/j.bbadis.2017.06.001. PubMed
  24. Fenoy IM, et al. 2015. Immunobiology. 22: 641-648. PubMed
  25. Jones C, et al. 2014. PLoS One. 9:90855. PubMed
  26. Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
  27. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  28. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: Phosphate-Buffered Saline (PBS), pH 7.4
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Type
Biology Area
Molecular Family
Gene ID
16153 View all products for this Gene ID
View information about IL-10 on UniProt.org

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Go To Top Version: 4    Revision Date: 09/12/2022

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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