- Regulatory Status
- Other Names
- CD41 Positive Selection, Human CD41 Positive Selection
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CD41+ expressing cells are either selected or depleted by incubating your sample with the directly conjugated CD41 Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD41+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.Product Details
- Kit Contents
For Cat# 480083:
- 1 vial containing 100 µl of Human CD41 Nanobeads
For Cat# 480084:
- 1 vial containing 1 ml of Human CD41 Nanobeads
- Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
- Conjugated Nanobeads: Protein-coated magnetic beads.
- Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
10 µl of Human CD41 Nanobeads for 1 x 107 cells in 100 µl of buffer. If the frequency of CD41+ cells is lower than 50%, or red cells are lysed prior magentic isolation, the volume of Nanobeads to be used needs to be titrated down.
- Application Notes
This kit is designed for the positive selection or depletion of human CD41+ cells from peripheral blood mononuclear cells (PBMCs).
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in columns: 8X
- Biology Area
- Molecular Family
- CD Molecules
- Gene ID
- View information about CD41 on UniProt.org
- Is there a way to detach your magnetic particles from the cell surface?
No, not currently. We have found that cells are functional without the need to detach the magnetic Nanobeads.
- What is the size of your magnetic particles?
The average diameter is approximately 130 nm.
- Can your magnetic particles be sterile filtered?
Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.