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MojoSort™ Buffer (5X)

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Regulatory Status
RUO
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Product Citations
publications
MojoSortBuffer_1_051215
A single cell suspension from pooled mouse spleen and lymph nodes was processed with MojoSort™ Buffer as indicated in the protocol for isolation of CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit with the MojoSort™ Magnet. The purity was checked by staining with PE anti-mouse CD4 (clone RM4-4) and APC anti-mouse CD3ε (clone 145-2C11).
  • MojoSortBuffer_1_051215
    A single cell suspension from pooled mouse spleen and lymph nodes was processed with MojoSort™ Buffer as indicated in the protocol for isolation of CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit with the MojoSort™ Magnet. The purity was checked by staining with PE anti-mouse CD4 (clone RM4-4) and APC anti-mouse CD3ε (clone 145-2C11).
  • MojoSortBuffer_2_051215
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480017 250 mL 53€
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Description

MojoSort™ Buffer is formulated with high quality reagents to help reduce non-specific binding when using the MojoSort™ Magnetic Separation System.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
The buffer should be stored between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. The 5X concentrate is filtered through a 0.2 µm membrane. Use the buffer under aseptic conditions as needed.

Application Notes

MojoSort™ Buffer is recommended for cell separation using MojoSort™ nanobeads and kits. To use the buffer, follow the protocol provided with the MojoSort™ product you are using.

Composition:

  1. 5X Phosphate buffered saline (PBS), pH 7.2
  2. 2.5 % (w/v) Bovine Serum Albumin (BSA)
  3. 10 mM Ethylene Diamine Tetra-acetic acid (EDTA)
Product Citations
  1. Knittel J, et al. 2022. STAR Protoc. 3:101632. PubMed
  2. Woodring T, et al. 2022. iScience. 25:104934. PubMed
  3. Xie L, et al. 2020. Infect Immun. :88. PubMed
  4. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  5. Lauko A, et al. 2022. Cell Rep. 40:111348. PubMed

Antigen Details

Gene ID
NA

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 1    Revision Date: 05/14/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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