LEGENDplex™ Mouse IL-5 Capture Bead A5, 13X

Pricing & Availability
Regulatory Status
RUO
Other Names
Mouse Th Cytokine Mix and Match Subpanel, Multiplex assay, Flow cytometry, Inflammation, T helper cells, Cytokines, Th1, Th2, Th9, Th17, Th1/Th2, Tfh, Th22
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publications
Mouse_IL-5_CB_1_101018

Data generated using the following LEGENDplex™ Mix & Match Reagents:

• Mouse IL-5 Capture Bead A5, 13X (Cat. # 740056)
• Mouse HSC Panel Detection Antibodies (Cat. # 740685)
• Mouse HSC Panel Standard (Cat. # 740684)
• Buffer Set H (Cat. # 740620)

Note: Data acquired on a BD FACSCalibur™ flow cytometer.

  • Mouse_IL-5_CB_1_101018

    Data generated using the following LEGENDplex™ Mix & Match Reagents:

    • Mouse IL-5 Capture Bead A5, 13X (Cat. # 740056)
    • Mouse HSC Panel Detection Antibodies (Cat. # 740685)
    • Mouse HSC Panel Standard (Cat. # 740684)
    • Buffer Set H (Cat. # 740620)

    Note: Data acquired on a BD FACSCalibur™ flow cytometer.

  • Mouse_IL-5_CB_2_101018

    Murine endothelial (CD31posCD41negCD45neg) and microglial cells (CD45posCD11bposF4/80pos) were isolated from digested E10.5 embryonic head tissue via FACS. Cells were co-cultured with OP9-DL1 cells for 7 days, and then culture supernatants were quantified using the LEGENDplex™ Mouse HSC Panel.

    Results are presented as the mean target concentration + SD (n = 6 embryos). Data were provided courtesy of Dr. Zhuan Li, MRC Centre for Inflammation Research, University of Edinburgh.

Cat # Size Price  
740056 100 tests (270 µL) 80,00€
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To purchase, view our LEGENDplex™ Mix and Match page

Description

This Mouse IL-5 Capture Bead is suitable for use with the following Mix and Match panels:

Mouse T Helper Cytokine Panel Version 3
741045 (LEGENDplex™ Mouse Th Panel Detection Antibodies V03)
741046 (LEGENDplex™ Mouse Th Panel Standard V03)
740368 (LEGENDplex™ Buffer Set A)

Mouse HSC Panel
740685 (LEGENDplex™ Mouse HSC Panel Detection Antibodies)
740684 (LEGENDplex™ Mouse HSC Panel Standard)
740620 (LEGENDplex™ Buffer Set H)

For product manuals for these panels, please visit the Mix and Match tab on the LEGENDplex™ page.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Application

Multiplex


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
16191 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 1    Revision Date: 07/18/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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