LEGENDplex™ Human Diabesity Panel - S/P (4-plex) with V-bottom Plate

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Regulatory Status
Other Names
PAI-1, Insulin, Leptin, Cortisol
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740914 100 tests 611€
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The Human Diabesity Panel is a multiplex bead-based assay panel, which uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows for the simultaneous quantification of 11 human proteins involved in diabesity, including PAI-1, GLP-1 (Total), Insulin, C-Peptide, TNFα, Glucagon, Leptin, Cortisol, IL-1β, IL-6 and GLP-1 (Active). This assay panel provides broader dynamic ranges than traditional ELISA methods and has been validated for use with tissue culture supernatant, serum, and EDTA plasma samples.


The LEGENDplex™ Human Diabesity Panel is configured as shown below depending on sample types and required dilutions:


Catalog No.

Plex Size


Recommended Sample Type

Recommended Dilution Factor




PAI-1, GLP-1 (Total), Insulin, C-Peptide, TNFα, Glucagon, Leptin, Cortisol*, IL-1β, IL-6 and GLP-1 (Active)

Tissue Culture





GLP-1 (Total), C-Peptide, TNFα, Glucagon, IL-1β, IL-6 and GLP-1 (Active)

Serum, Plasma





PAI-1, Insulin, Leptin, Cortisol*

Serum, Plasma




* This panel introduces a competitive assay for Cortisol (on B5 bead). A concentrated cortisol tracer is provided in this kit which will need to be diluted 100-fold before the assay. This cortisol tracer will be added to all wells before adding the beads. The competition between the cortisol tracer and the standard cortisol will characterize the standard curve. Due to the nature of the assay, the standard curve will have the highest signal at the lowest standard cortisol concentration and will decrease as the standard cortisol concentration increases.

Note: The cortisol tracer is for the cortisol assay ONLY and does not affect any other target.

The LEGENDplex™ Human Diabesity Panel is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.

This assay is for research use only.

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads 
  • Setup Beads: Raw Beads 
  • Capture Beads 
  • Detection Antibodies 
  • Standard Cocktail, Lyophilized 
  • Human Cortisol Tracer, 100X 
  • SA-PE 
  • Assay Buffer 
  • Matrix B, Lyophilized 
  • Wash Buffer, 20X 
  • Plate Sealers 
  • V-bottom Plate

Product Details

Verified Reactivity


Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Micro FACS tubes (if the flow cytometer does not contain an autosampler)
  • Vortex Mixer
  • Sonicator
  • Aluminum Foil
  • Absorbent Pads or Paper Towels
  • Plate Shaker
  • Tabletop Centrifuges
  • A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
  • Manual

Antigen Details

Biology Area
Molecular Family
Cytokines/Chemokines, Enzymes and Regulators
Gene ID
5054 View all products for this Gene ID 3630 View all products for this Gene ID 3952 View all products for this Gene ID

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 4    Revision Date: 06/15/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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