LEGEND MAX™ Human IL-17A ELISA Kit

Pricing & Availability
Other Names
IL-17A ELISA Kit, IL-17 ELISA Kit
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Product Citations
publications
IL-17A_Human_LegMax_110111
  • IL-17A_Human_LegMax_110111
Cat # Size Price Quantity Save
433917 1 Pre-coated Plate 280€
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Description

IL-17 or IL-17A originally identified as cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) in mouse, is a founding member of the IL-17 family of cytokines. IL-17A is mainly produced by a small subset of T helper cells called Th17 cells. IL-17A is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with a molecular mass of 35 kD. The amino acid sequence of human IL-17A is 63% and 58% identical to mouse and rat IL-17A, respectively, and 72% identical to the ORF13 of Herpesvirus saimiri. IL-17A exhibits multiple biological activities including the induction of various proinflammatory cytokines and chemokines such as IL-6, IL-8, G-CSF, GM-CSF, GRO-a, and MCP-1 on a variety of cell types. IL-17A is also involved in the activation of NF-kB and costimulation of T cell proliferation. As a result of these roles, IL-17A has been linked to many immune/autoimmune related diseases including rheumatoid arthritis, asthma, lupus, and multiple sclerosis.


BioLegend’s LEGEND MAX™ Human IL-17A ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is precoated with a capture antibody. This kit is specifically designed for the accurate protein quantitation of human IL-17A from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human IL-17A Pre-coated 96-well Strip Microplate
  • Human IL-17A Dectection Antibody
  • Human IL-17A Standard
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Verma NK, et al. 2012. J. Biol Chem. 287:27204. PubMed.
Product Citations
  1. Verma N, et al. 2012. J Biol Chem. 287: 27204. PubMed
  2. Moafi M, et al. 2017. Scand J Immunol. 85:291. PubMed
  3. Schwarzkopf K, et al. 2019. Hepatol Commun. 3:392. PubMed
Publication Library
Sensitivity
0.8 pg/mL
Standard Range
3.9-250 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated T cell subpopulation
Biology Area
Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3605 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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