Go-ChIP-Grade™ Purified Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
27-35 (See other available formats)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
27-35_GoChIP_IgG2b-k_ctrl_1_060618_updated
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone 27-35, Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 27-35_GoChIP_IgG2b-k_ctrl_1_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone 27-35, Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 27-35_GoChIP_IgG2b-k_ctrl_2_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone 27-35, Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Cat # Size Price Quantity Avail. Save
402211 25 µg 24€
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402212 100 µg 68€
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Description

Clone 27-35 is a mouse IgG2b, κ isotype control which was generated in mice using Dansyl-Keyhole Limpet Hemocyanin (DNS-KLH) immunogen. It does not have any tissue-associated epitope and has been screened against human and mouse tissue to assure its unspecificity and low background.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Dansyl-Keyhole Limpet Hemocyanin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP assay as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Application References

(PubMed link indicates BioLegend citation)
  1. Prussin C, et al. 1995. J. Immunol. Methods 188:117.

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Go To Top Version: 1    Revision Date: 05/24/2018

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