Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG2a-53 (See other available formats)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
MG2a-53_GoChIP_IgG2a_Iso-Con_060618_updated
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2a-53_GoChIP_IgG2a_Iso-Con_060618_updated
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Cat # Size Price Quantity Avail. Save
401505 25 µg 24€
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401506 100 µg 68€
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Description

The isotype of MG2a-53 immunoglobulin is mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Application Notes

The MG2a-53 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 401504) as negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149.
  2. Lee H, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:2885. PubMed
  3. Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?
IgG2a gene is deleted in C57Bl/6, C57Bl/ 10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Go To Top Version: 1    Revision Date: 05/14/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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