- P83B5 (See other available formats)
- Regulatory Status
- Other Names
- Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to a cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Purified recombinant fragment (aa 20-240) of human STAT3 expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
- The Go-ChIP-Grade™ Purified antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ChIP - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by ChIP. For ChIP, the suggested use of this reagent is 1-50-1:150 dilution by volume. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The canonical sequence of STAT3 contains 770 amino acids with a predicted molecular weight of 88 kD. A shorter isoform of STAT3 with 722 amino acids (83 kD) was reported. Clone P83B5 may recognize both isoforms.
25 µg, 100 µg of Go-ChIP-Grade Purified™ antibody can be used for 2-8, 11-33 immunoprecipitations, respectively, at the recommended dilution.
(PubMed link indicates BioLegend citation)
- Akira S, et al. 1994. Cell. 77:63.
- Zhang X, et al. 1995. Science 267:1990.
- Sanchez-Margalet V & Martin-Romero C. 2001. Cell Immunol. 211:30.
- Simon A, et al. 2000. Science 290:144.
- Hoey T & Grusby MJ. 1999. Adv. Immunol. 71:145.
- Product Citations
AB_2716049 (BioLegend Cat. No. 691503)
AB_2716050 (BioLegend Cat. No. 691504)
- STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to the nucleus to act as a transcriptional activator, activates a wide variety of genes involved in cell growth and apoptosis, and is involved in gp130-related signaling.
- STAT3 forms a homodimer or a heterodimer with other STAT family members and interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
- Cell Type
- Embryonic Stem Cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Akira S, et al. 1994. Cell. 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V & Martin-Romero C. 2001. Cell Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T & Grusby MJ. 1999. Adv. Immunol. 71:145.
- Gene ID
- 6774 View all products for this Gene ID
- View information about STAT3 on UniProt.org
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