Go-ChIP-Grade™ Purified anti-HDAC7 Antibody

Pricing & Availability
Clone
11C7C49 (See other available formats)
Other Names
Histone deacetylase 7, HD7, HDAC7a, HDAC 7, HDAC-7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
11C7C49_GoChIP_HDAC7_Antibody_042617
Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC7 Antibody (Clone 11C7C49), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-HDAC7 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human NucE gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 11C7C49_GoChIP_HDAC7_Antibody_042617
    Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-HDAC7 Antibody (Clone 11C7C49), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-HDAC7 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human NucE gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 11C7C49_GoChIP_HDAC7_Antibody_WB_042717
    Total lysates (15 µg protein) from HeLa (lane 1), Jurkat (lane 2) and NIH3T3 cells (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-HDAC7 Antibody, clone 11C7C49 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC7 Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
683503 25 µg 60€
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683504 100 µg 188€
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Description

Histone deacetylases (HDACs) regulate a variety of biological processes by deacetylating lysine residues in the N-terminal tails of core histones and non-histone proteins. HDAC7 is a class IIa histone deacetylase and its enzymatic activity is dependent on the interaction with HDAC3. HDAC7 is highly expressed in CD4+CD8double-positive thymocytes and has been suggested to repress the expression of Nur77 through the transcription factor MEF2D. HDAC7 is mostly localized to the nucleus and can be sequestrated in the cytoplasm by interacting with 14-3-3 protein during T cell receptor activation. The nuclear export of HDAC7 leads to the expression of Nur77 and apoptosis. HDAC7 has been shown to regulate the maturation of osteoclast and osteoblast, as well as the development of muscle and blood vessel.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic HDAC7 peptide conjugated to KLH (1-17 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. 

It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

25 µg, 100 µg of Go-ChIP-GradeTM Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution.

RRID
AB_2687137 (BioLegend Cat. No. 683503)
AB_2687138 (BioLegend Cat. No. 683504)

Antigen Details

Structure
952 amino acids with a predicted molecular weight of 102.9 kD. Contains a histone deacetylase domain.
Distribution

Cytoplasm and the nucleus.

Function
HDAC7 mediates deacetylation of lysine residues in the N-terminal tails of core histones. It plays important roles in the regulation of T-cell differentiation and development of muscle and blood vessel.
Interaction
HDAC7 interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, FOXP3, KAT5, EDNRA, KDM5B, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Barneda-Zahonero B, et al. 2013. PLoS Genet. 9:e1003503.
2. Pham L, et al. 2011. J. Biol. Chem. 286:12056.
3. Li S, et al. 2011. Mol. Cancer 10:18.
4. Gao C, et al. 2010. Biochim. Biophys. Acta. 1803:1186.
5. Margariti A, et al. 2009. J. Cell. Sci. 122:460.
6. Parra M, et al. 2007. Genes Dev. 21:638.

Gene ID
51564 View all products for this Gene ID
UniProt
View information about HDAC7 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/04/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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