GMP Recombinant Human M-CSF (carrier-free)

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Other Names
Colony Stimulating Factor 1, Colony Stimulating Factor 1 (Macrophage), Macrophage Colony Stimulating Factor 1, CSF-1, MCSF, CSF1
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GMP recombinant human M-CSF induces proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells in a dose-dependent manner with an ED50 range of 0.5 - 2.0 ng/mL.
  • Human_MCSF_CF_GMP-RECOM_061020.png
    GMP recombinant human M-CSF induces proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells in a dose-dependent manner with an ED50 range of 0.5 - 2.0 ng/mL.
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574814 25 µg 236€
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574816 100 µg 740€
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Description

Macrophage colony formation from precursors in murine bone marrow cultures. M-CSF is constitutively present at biologically active concentrations in human serum. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, cytotoxicity for tumor cells as well as synthesis of inflammatory cytokines such as IL-1, TNF-α, and INF-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts; consequently, M-CSF is a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces VEGF in human monocytes in human tumors; high levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer. High levels of M-CSF have been associated with different pathologies such as pulmonary fibrosis and atherosclerosis. M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Human M-CSF and IL-34 exhibit cross-species specificity – both bind to human and mouse M-CSF receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human M-CSF, amino acids Glu33-Ser190 (Accession# NM_172212.2) was expressed in 293E cells.
Molecular Mass
The 179 amino acid recombinant protein has a predicted molecular mass of approximately 20.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 25 - 35 kD and 55 - 70 kD respectively by SDS-PAGE. The N-terminal contains a His9-(SGGG)2-IEGR-tag.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.1 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method
Concentration
500 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.5 - 2.0 ng/mL as measured by its ability to induce proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells.
Application

Bioassay
Cell cultures

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

 

Disclaimer

GMP Recombinant Proteins. BioLegend GMP recombinant proteins are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures. Our processes include:

  • Batch-to-batch consistency
  • Material traceability
  • Documented procedures
  • Documented employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products

BioLegend GMP recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12.

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Hematopoietic stem and progenitors, embryonic stem cells

Function
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Interaction
Monocytes, macrophages, mononuclear phagocyte precursors, microglia, proliferating smooth muscle cells, umbilical vein endothelial cells, and breast cancer cell lines
Ligand/Receptor
M-CSFR or CSF1R (CD115)
Bioactivity
Measured by its ability to induce proliferation of M-NFS-60 cells.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors, Growth Factors
Antigen References

 

  1. Kawasaki ES, et al. 1985. Science 230:291.
  2. Wei S, et al. 2010. J. Leukoc. Biol. 88:495.
  3. Hodge JM, et al. 2011. PloS One 6:e21462.
  4. Morandi A, et al. 2011. PloS One 6:e27450.
  5. Erblich B, et al. 2011. PloS One 6:e26317.
  6. MacDonald KP, et al. 2010. Blood 116:3955.
Gene ID
1435 View all products for this Gene ID
UniProt
View information about M-CSF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/16/2020

For research and ex vivo cell processing use. Not for diagnostic or therapeutic procedures. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to reverse engineer functionally similar materials without written approval of BioLegend. By use of these products you accept the terms and conditions of any and all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research and ex vivo cell processing use only and not intended for human or animal diagnostic or therapeutic use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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