- Clone
- HIB19 (See other available formats)
- Workshop
- V CD19.11
- Other Names
- B4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Typical results from human peripheral blood lymphocytes stained either with HIB19 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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260154 | 100 tests | 331€ |
CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 50 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).
Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing
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Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Bradbury L, et al. 1993. J. Immunol. 151:2915.
- Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
- Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
- Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- RRID
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AB_2924511 (BioLegend Cat. No. 260154)
- Disclaimer
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GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 95 kD
- Distribution
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B lineage (except plasma cells), follicular dendritic cells
- Function
- B cell activation and differentiation
- Ligand/Receptor
- Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915. - Gene ID
- 930 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Related FAQs
Other Formats
View All CD19 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD19
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Biotin anti-human CD19
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FITC anti-human CD19
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PE anti-human CD19
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PE/Cyanine5 anti-human CD19
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Purified anti-human CD19
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APC/Cyanine7 anti-human CD19
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PE/Cyanine7 anti-human CD19
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Alexa Fluor® 488 anti-human CD19
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Alexa Fluor® 647 anti-human CD19
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Pacific Blue™ anti-human CD19
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Alexa Fluor® 700 anti-human CD19
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PerCP anti-human CD19
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PerCP/Cyanine5.5 anti-human CD19
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Brilliant Violet 421™ anti-human CD19
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Brilliant Violet 570™ anti-human CD19
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Brilliant Violet 650™ anti-human CD19
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Brilliant Violet 785™ anti-human CD19
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Brilliant Violet 510™ anti-human CD19
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Brilliant Violet 605™ anti-human CD19
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Brilliant Violet 711™ anti-human CD19
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Purified anti-human CD19 (Maxpar® Ready)
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Alexa Fluor® 594 anti-human CD19
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PE/Dazzle™ 594 anti-human CD19
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PE anti-human CD19
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APC/Fire™ 750 anti-human CD19
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Pacific Blue™ anti-human CD19
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APC anti-human CD19
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PE/Cyanine7 anti-human CD19
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TotalSeq™-A0050 anti-human CD19
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Brilliant Violet 750™ anti-human CD19
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TotalSeq™-B0050 anti-human CD19
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TotalSeq™-C0050 anti-human CD19
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PerCP/Cyanine5.5 anti-human CD19
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Spark NIR™ 685 anti-human CD19
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Ultra-LEAF™ Purified anti-human CD19
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APC/Fire™ 810 anti-human CD19
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PE/Fire™ 640 anti-human CD19
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PE/Fire™ 700 anti-human CD19
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TotalSeq™-D0050 anti-human CD19
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Spark YG™ 593 anti-human CD19
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GMP Pacific Blue™ anti-human CD19
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Spark Violet™ 423 anti-human CD19
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GMP PE anti-human CD19
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GMP APC anti-human CD19
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KIRAVIA Blue 520™ anti-human CD19
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GMP PerCP/Cyanine5.5 anti-human CD19
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GMP PE/Cyanine7 anti-human CD19
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Spark Violet™ 500 anti-human CD19
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