GMP PE/Cyanine7 anti-human CD19, CD19, HIB19

Pricing & Availability

Clone: HIB19 (See other available formats)
Workshop: V CD19.11
Other Names: B4
Isotype: Mouse IgG1, κ
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Product Citations: publications

HIB19_GMP-PECyanine7_Antibody_082622 — Typical results from human peripheral blood lymphocytes stained either with HIB19 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

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260154

100 tests

331€

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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

50 µg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁸ and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Bradbury L, et al. 1993. J. Immunol. 151:2915.
Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

RRID

AB_2924511 (BioLegend Cat. No. 260154)

Disclaimer

GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:

Batch-to-batch consistency
Material traceability
Documented procedures
Documented employee training
Equipment maintenance and monitoring records
Lot-specific certificates of analysis
Quality audits per ISO 13485:2016
QA review of released products

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution: B lineage (except plasma cells), follicular dendritic cells
Function: B cell activation and differentiation
Ligand/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930 View all products for this Gene ID
UniProt: View information about CD19 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD19	HIB19	FC
Biotin anti-human CD19	HIB19	FC
FITC anti-human CD19	HIB19	FC
PE anti-human CD19	HIB19	FC
PE/Cyanine5 anti-human CD19	HIB19	FC
Purified anti-human CD19	HIB19	FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19	HIB19	FC
PE/Cyanine7 anti-human CD19	HIB19	FC
Alexa Fluor® 488 anti-human CD19	HIB19	FC,ICC
Alexa Fluor® 647 anti-human CD19	HIB19	FC,IHC-F
Pacific Blue™ anti-human CD19	HIB19	FC
Alexa Fluor® 700 anti-human CD19	HIB19	FC
PerCP anti-human CD19	HIB19	FC
PerCP/Cyanine5.5 anti-human CD19	HIB19	FC
Brilliant Violet 421™ anti-human CD19	HIB19	FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19	HIB19	FC
Brilliant Violet 650™ anti-human CD19	HIB19	FC
Brilliant Violet 785™ anti-human CD19	HIB19	FC
Brilliant Violet 510™ anti-human CD19	HIB19	FC
Brilliant Violet 605™ anti-human CD19	HIB19	FC
Brilliant Violet 711™ anti-human CD19	HIB19	FC
Purified anti-human CD19 (Maxpar® Ready)	HIB19	FC,CyTOF®
Alexa Fluor® 594 anti-human CD19	HIB19	ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19	HIB19	FC
PE anti-human CD19	HIB19	FC
APC/Fire™ 750 anti-human CD19	HIB19	FC
Pacific Blue™ anti-human CD19	HIB19	FC
APC anti-human CD19	HIB19	FC
PE/Cyanine7 anti-human CD19	HIB19	FC
TotalSeq™-A0050 anti-human CD19	HIB19	PG
Brilliant Violet 750™ anti-human CD19	HIB19	FC
TotalSeq™-B0050 anti-human CD19	HIB19	PG
TotalSeq™-C0050 anti-human CD19	HIB19	PG
PerCP/Cyanine5.5 anti-human CD19	HIB19	FC
Spark NIR™ 685 anti-human CD19	HIB19	FC
Ultra-LEAF™ Purified anti-human CD19	HIB19	FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19	HIB19	FC
PE/Fire™ 640 anti-human CD19	HIB19	FC
PE/Fire™ 700 anti-human CD19	HIB19	FC
TotalSeq™-D0050 anti-human CD19	HIB19	PG
Spark YG™ 593 anti-human CD19	HIB19	FC
GMP Pacific Blue™ anti-human CD19	HIB19	FC
Spark Violet™ 423 anti-human CD19	HIB19	FC
GMP PE anti-human CD19	HIB19	FC
GMP APC anti-human CD19	HIB19	FC
KIRAVIA Blue 520™ anti-human CD19	HIB19	FC
GMP PerCP/Cyanine5.5 anti-human CD19	HIB19	FC
GMP PE/Cyanine7 anti-human CD19	HIB19	FC
Spark Violet™ 500 anti-human CD19	HIB19	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/19/2022

For research use only. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.