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FITC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_FITC_090507
C57BL/6 mouse splenocytes stained with 104 FITC
  • 104_FITC_090507
    C57BL/6 mouse splenocytes stained with 104 FITC
Compare all formats See FITC spectral data
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109805 50 µg 44€
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109806 500 µg 93€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  3. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  4. Michalova J, et al. 2011. Biol Blood Marrow Transplant. 17:1273. PubMed
  5. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  6. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  7. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  8. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  9. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  10. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  11. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  12. Burman A, et al. 2007. Blood. 110:1064. PubMed
  13. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  14. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  15. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  16. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  17. Liang Y, et al. 2020. J Immunol. 204:2984. PubMed
  18. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  19. Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed
  20. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  21. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  22. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  23. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  24. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  25. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  26. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  27. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  28. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  29. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  30. Schulz A, et al. 2019. Cell Rep. 29:1074. PubMed
  31. Guiducci E, et al. 2018. Front Immunol. 1.467361111. PubMed
  32. Zhang J, et al. 2022. iScience. 25:104963. PubMed
  33. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  34. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  35. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  36. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  37. Li J, et al. 2020. Elife. 9:00. PubMed
  38. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  39. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  40. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  41. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  42. Wan X, et al. 2018. Nature. 560:107. PubMed
  43. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  44. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  45. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  46. Faulhaber LD, et al. 2022. Neurophotonics. 9:031917. PubMed
  47. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  48. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  49. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  50. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  51. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  52. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  53. Leon-Rico D, et al. 2015. Lab Anim. 49:132. PubMed
  54. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  55. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  56. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  57. Uslu M, et al. 2020. J Cell Physiol. 235:9644. PubMed
  58. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  59. Burgess M, et al. 2014. Blood. 124:3947. PubMed
  60. Kesavardhana S, et al. 2020. J Biol Chem. 295:8325. PubMed
  61. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  62. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
  63. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  64. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  65. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  66. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  67. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  68. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  69. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  70. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  71. Jaako P, et al. 2016. Leukemia. 10.1038/leu.2016.159. PubMed
  72. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  73. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  74. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  75. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  76. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  77. Peter Morawski, Chen-Feng Qi, Silvia Boll 2017. Sci Rep. 7:40838. PubMed
  78. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  79. Düking T, et al. 2022. Sci Adv. 8:eabo7639. PubMed
  80. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  81. Koide S, et al. 2022. iScience. 25:103603. PubMed
  82. Okuyama Y, et al. 2020. FASEB J. 540:34. PubMed
  83. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  84. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  85. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  86. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  87. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  88. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  89. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  90. Zhang J, et al. 2019. Exp Hematol. 78:21:00. PubMed
  91. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  92. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  93. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  94. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  95. Stienstra R, et al. 2014. Diabetes. 63:4143. PubMed
  96. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  97. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  98. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  99. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  100. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  101. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  102. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  103. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_313442 (BioLegend Cat. No. 109805)
AB_313443 (BioLegend Cat. No. 109806)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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