FITC anti-mouse CD40 Antibody

Pricing & Availability
HM40-3 (See other available formats)
Regulatory Status
Other Names
Armenian Hamster IgM
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Product Citations
C57BL/6 mouse splenocytes stained with HM40-3 FITC
  • HM40-3_FITC_090507
    C57BL/6 mouse splenocytes stained with HM40-3 FITC
Compare all formats See FITC spectral data
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102905 50 µg 67€
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102906 500 µg 229€
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CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. The HM40-3 antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Armenian Hamster
(BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD40 ligand binding1, stimulation of mouse B cell and dendritic cell proliferation2-6,9, and immunohistochemistry7 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102908).

Application References

(PubMed link indicates BioLegend citation)
  1. Kawano T, et al. 1997. Science 278:1626. (Block)
  2. Kaneko Y, et al. 1996. Eur. J. Immunol. 26:3061. (Costim)
  3. Kashiwada M, et al. 1996. Eur. J. Immunol. 26:1451. (Costim)
  4. Inaba M, et al. 1995. Eur. J. Immunol. 25:1244. (Costim)
  5. Akiba H, et al. 1999. J. Immunol. 162:7058. (Costim)
  6. Ridge JP, et al. 1998. Nature 393:474. (Costim)
  7. Morelli AE, et al. 2000. Transplantation 69:2647. (IHC)
  8. Ma XT, et al. 2006. Cancer Research 66:1169.
  9. Yu X, et al. 2006. J. Biol. Chem. 281:15505. (Costim)
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  2. Regli IB, et al. 2020. Cell Reports. 31(10):107746. PubMed
  3. Salvi V, et al. 2021. JCI Insight. 6:. PubMed
AB_312948 (BioLegend Cat. No. 102905)
AB_312949 (BioLegend Cat. No. 102906)

Antigen Details

TNFR superfamily, 48 kD

B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors

Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
CD40L (CD154)
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21939 View all products for this Gene ID
View information about CD40 on

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Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD40

  • Purified anti-mouse CD40

  • Alexa Fluor® 488 anti-mouse CD40

  • Alexa Fluor® 647 anti-mouse CD40

  • Ultra-LEAF™ Purified anti-mouse CD40


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