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FITC anti-mouse CD25 Antibody

Pricing & Availability
Clone
PC61 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG1, λ
Ave. Rating
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Product Citations
publications
PC61_FITC_111407
Con-A Stimulated (3 days) BALB/c mouse splenocytes stained with PC61 FITC
  • PC61_FITC_111407
    Con-A Stimulated (3 days) BALB/c mouse splenocytes stained with PC61 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
102005 50 µg 57€
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102006 500 µg 203€
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody (Cat. No. 101902). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102040) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
  2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
  3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
  4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
  5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
  6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
  7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
  8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
  9. León-Ponte M, et al. 2007. Blood 109:3139.
  10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
  11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.
  12. Liu F, et al. 2011. Arch Toxicol. 85:1383. PubMed
  13. Anguela XM, et al. 2013. Diabetes. 62:551. PubMed
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Schuster M, et al. 2017. J Immunol. 199:920. PubMed
  3. Fujimori S, et al. 2022. Elife. 11:. PubMed
  4. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  5. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  6. Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed
  7. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
  8. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  9. Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed
  10. Uchida A, et al. 2021. J Inflamm Res. 14:3089. PubMed
  11. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  12. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  13. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  14. Pu Y, et al. 2022. Transl Psychiatry. 12:223. PubMed
  15. Carbonnelle D, et al. 2009. J Pharmacol Exp Ther. 331:771. PubMed
  16. Wan X, et al. 2018. Nature. 560:107. PubMed
  17. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  18. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  19. Naik A, et al. 2015. J Immunol. 194:790. PubMed
  20. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  21. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  22. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  23. Kojima H, et al. 2021. Scand J Immunol. 93:e13020. PubMed
  24. Thornton T, et al. 2016. Nat Commun. 7:10553. PubMed
  25. Moon J, et al. 2020. Immune Netw. 20:e40. PubMed
  26. Schuster M, et al. 2019. Front Immunol. 10:1583. PubMed
  27. Li J, et al. 2021. Cell Rep. 37:110124. PubMed
  28. Le QV, et al. 2022. Bioact Mater. 15:160. PubMed
  29. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
  30. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  31. Amantini C, et al. 2017. Oncotarget. 8:90766. PubMed
  32. Toubaji A, et al. 2007. Vaccine. 25:5882. PubMed
  33. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
RRID
AB_312854 (BioLegend Cat. No. 102005)
AB_312855 (BioLegend Cat. No. 102006)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Taniguchi T, et al. 1993. Cell 73:5-8.
  2. Waldmann TA. 1991. J Biol Chem. 266:2681-4.
  3. Read S, et al. 2000. J Exp Med. 192:295-302.
  4. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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