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FITC anti-human Podocalyxin Antibody

Pricing & Availability
Clone
mAb 84 (See other available formats)
Regulatory Status
RUO
Other Names
GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1, GCTM2
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
mAb84_FITC_Podocalyxin_Antibody_071918
Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and anti-human Podocalyxin (clone mAb 84, left) FITC or Mouse IgM, κ isotype control FITC (right).
  • mAb84_FITC_Podocalyxin_Antibody_071918
    Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and anti-human Podocalyxin (clone mAb 84, left) FITC or Mouse IgM, κ isotype control FITC (right).
Compare all formats See FITC spectral data
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374303 25 tests 104€
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374304 100 tests 221€
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Description

Podocalyxin-like protein-1 (also known as PODXL, GP200, PCLP1 and GCTM2) is expressed on the surface of undifferentiated human ES cells and glomerular podocytes. PODXL was originally described as the main sialoprotein expressed by glomerular epithelial cells. Recently PODXL been associated with several forms of cancer and is being targeted as a possible therapeutic. Clone mAb 84 is reported to recognize and have cytotoxic affects against undifferentiated human ES cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats) include: WB, IP, cytotoxicity. The LEAF purified format is recommended for cytotoxicity assays. 

RRID
AB_2749881 (BioLegend Cat. No. 374303)
AB_2749882 (BioLegend Cat. No. 374304)

Antigen Details

Structure
Sialomucin family
Distribution

Stem cells, Glomerular podocytes

Function
Stem cell differentiation, regulatory factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References

1. Kerjaschki D, et al. 1984. J Cell Bio. 98:1591.
2. Horvat R, et al. 1986. J Cell Bio. 102:484.
3. Choo AB, et al. 2008. Stem Cells. 26:1454.
4. Tan HL, et al. 2009. Stem Cells. 27:1792.
5. Nielsen JS, et al. 2009. J Am Soc Nephrol. 20:1669.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about Podocalyxin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/19/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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