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This antibody is effective in immunohistochemistry (IHC). This antibody has been used successfully on frozen sections.
Positive Control Tissue: Normal skin
This product may contain other non-IgG subtypes.
- Product Information
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The polyclonal antibody Poly19058 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Filaggrin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- This monospecific polyclonal antibody was raised against a peptide corresponding to residues 24-39 in the partial sequence of filaggrin.
- Phosphate-buffered solution+ 0.03% Thimerosal.
- The antibody was purified by peptide affinity chromatography.
- 1.0 mg/mL
- Storage & Handling
- When stored at ≤ -20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 24 months from the date of thaw or until the expiry date on the label, whichever comes first.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: 1:500 with either biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: Frozen tissue sections
Pretreatment: Not Required
Incubation: 60 minutes at room temperature
(PubMed link indicates BioLegend citation)
- Wallace L, Roberts-Thompson L, and Reichelt J. Deletion of K1/K10 does not impair epidermal stratification but affects desmosomal structure and nuclear integrity. J. Cell Sci., 125: 1750-1758, Apr 2012. PubMed
- Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and PTrez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. [IHC] PubMed
- Hu Y, Baud V, Oga T, Kim KI, Yoshida K, Karin M. IKKalpha controls formation of the epidermis independently of NF-kappaB. Nature 410:710-714, 2001.
- Yuspa SH, Kilkenny AD, Steinert PM, Roop DR. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in-vitro. J Cell Biol 109:1207-1217, 1989.
- Roop DR, Cheng CK, Titterington L, Meyers CA, Stanley JR, Steinert PM, Yuspa SH. Synthetic peptides corresponding to keratin subunits elicit highly specific antibodies. J Biol Chem 259:8037-8040, 1984.
- Liang Y. 2011. Patholog Res Int. 2011:93674. (IHC) PubMed
- DiTommaso T, et al. 2014. PLoS Genet. 10:1004706. (IHC) PubMed
- Liakath-Ali K, et al. 2014. Nat Commun. 11:3540. (IHC) PubMed
- Product Citations
AB_2565053 (BioLegend Cat. No. 905801)
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
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