Epitope Tag Small Motif Antibody Sampler Kit

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A_SmallMotifSampler_DYKDDDDK_WB_072717
50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-DYKDDDDK Tag (clone L5) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder. Lane M*: long exposure MW ladder.
  • A_SmallMotifSampler_DYKDDDDK_WB_072717
    50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-DYKDDDDK Tag (clone L5) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder. Lane M*: long exposure MW ladder.
  • B_SmallMotifSampler_His-Tag_WB_072717
    50ng recombinant human IFN-γ protein (lane 1, negative control) and Posi-Tag Epitope Tag Protein (lane 2) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.2 µg/mL Purified anti-His Tag (clone J099B12) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Lane M: MW ladder.
  • C_SmallMotifSampler_HA11_WB_072717
    15µg each of total protein from CHO cells (lane 3), and CHO transfected with HA tagged protein (lane 4) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL Purified anti-HA.11 Epitope Tag (clone 16B12) Antibody. Proteins were visualized using an HRP goat anti-mouse IgG (clone Poly4053) secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) Antibody was used as a loading control. Lane M: MW ladder. Lane M*: long exposure MW ladder.
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Description

The Epitope Tag (Small Motif) Antibody Sampler Kit provides a convenient resource to detect small Epitope Tag fused protein by Western Blot. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. Anti-His tag antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. The 9E10 monoclonal antibody recognizes human myc and the 10 amino acid epitope tag of human c-myc. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. For additional antigen details, please refer to individual product datasheets.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD)
Anti-DYKDDDDK L5 25 µg DYKDDDDK tag epitope Rat IgG2a, κ N/A
Anti-c-Myc 9E10 25 µg EQKLISEEDL tag epitope Mouse IgG1 N/A
Anti-HA.11 16B12 25 µg YPYDVPDYA tag epitope Mouse IgG1, κ N/A
Anti-V5 Tag 7/4 25 µg GKPIPNPLLGLDST tag epitope Mouse IgG N/A
Anti-His Tag J099B12 25 µg 6x His tag epitope Mouse IgG1, κ N/A

* For detailed information about each specificity, please follow the provided links. 

Product Details

Formulation
Please refer to individual product datasheets for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Concentration
All antibodies in the kit are at 0.5mg/ml
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-DYKDDDDK: 0.1-1 µg/ml
Anti-Myc: 0.1-0.5 µg/ml
Anti-HA.11: 0.1-1 µg/ml
Anti-V5 tag: 0.1-0.5 µg/ml
Anti-His tag: 0.1-0.2 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed (Anti-DYKDDDDK)
  2. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed (Anti-DYKDDDDK)
  3. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed (Anti-DYKDDDDK)
  4. Das A, et al. 2016. J. Biol. Chem.. 291: 6096 - 6110. PubMed (Anti-DYKDDDDK)
  5. Kondo, S., et al. 2011. J. Virol. 85:11255. PubMed (Anti-Myc)
  6. Bourgeois-Daigneault MC, et al. 2012. J Immunol. 188:4959. PubMed (Anti-Myc)
  7. Robu M, et al. 2013. PNAS. 110:1658. PubMed (Anti-Myc)
  8. Pecot MY, Malhotra V. 2004. Cell. 116:99. (Anti-HA.11)
  9. Görtz D, et al. 2015. Scientific Reports. 5: 14685. PubMed (Anti-HA.11)
  10. Thoms M, et al. 2016. Nucleic Acids Res. 44: 926 - 939. PubMed (Anti-HA.11)
  11. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974. (Anti-His tag)
  12. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429. (Anti-His tag)
  13. Keshwani M, et al. 2015. Biochem. J.. 472: 329 - 338. PubMed (Anti-His tag)
Product Citations

Antigen Details

Biology Area
Cell Biology
Gene ID
4609 View all products for this Gene ID

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Recombinant Mouse CXCL7 (NAP-2) (carrier-free) BA
Recombinant Mouse CXCL3 (carrier-free) BA
Recombinant Mouse CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL23 (MPIF-1) (Arg46-Asn120) (carrier-free) BA
Recombinant Human Erythropoietin (EPO) (carrier-free) BA
Recombinant Mouse IL-25 (IL-17E) (insect expressed, carrier-free) BA
Recombinant Human FASL (TNFSF6) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) ELISA
Recombinant Mouse CXCL14 (BRAK) (carrier-free) BA
Recombinant Human CXCL14 (BRAK) (carrier-free) BA
Recombinant Mouse Erythropoietin (EPO) (carrier-free) BA
Recombinant Human CXCL12 (SDF-1β) (carrier free) BA
Recombinant Mouse CCL19 (MIP-3β) (carrier-free) BA
Recombinant Mouse CCL12 (MCP-5) (carrier-free) BA
Zombie NIR™ Fixable Viability Kit FC, ICFC
Recombinant Human VEGF-C (carrier-free) BA
Recombinant Mouse NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Human NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Mouse IGF-II (carrier-free) BA
Purified Annexin V FC
Recombinant Mouse CXCL12 (SDF-1β) (carrier-free) BA
Recombinant Human TGF-α (carrier-free) BA
Recombinant Rat CCL20 (MIP-3α) (carrier-free) BA
LEGEND MAX™ Human CCL17 (TARC) ELISA Kit ELISA
LEGEND MAX™ Human CCL5 (RANTES) ELISA Kit ELISA
Recombinant Mouse CXCL4 (PF-4) (carrier-free) BA
Accutase® Cell Detachment Solution FC
Recombinant Human IGF-II (carrier-free) BA
Recombinant Human IGF-I (carrier-free) BA
Recombinant Human TRANCE (RANKL) (carrier-free) BA
Recombinant Mouse BAFF (carrier-free) BA
Recombinant Human APRIL (TNFSF13) (ELISA Std.) ELISA
Recombinant Human IL-15 (ELISA Std.) ELISA
Recombinant Mouse IGF-I (carrier-free) BA
Recombinant Human sTNF-RII (TNFRSF1B) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL17 (TARC) ELISA
Recombinant Mouse CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Human CD40L (TNFSF5) (carrier-free) BA
Recombinant Human CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Mouse TNF-α (Animal-Free) BA
Recombinant Human Activin A (carrier-free) BA
Recombinant Human FGF-4 (carrier-free) BA
Recombinant Human IL-18 (carrier-free) BA
Recombinant Rat GM-CSF (carrier-free) BA
Recombinant Human IFN-α2 (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL5 (RANTES) ELISA
ELISA MAX™ Deluxe Set Human CXCL5 ELISA
LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit ELISA
ELISA MAX™ Deluxe Set Human CCL28 ELISA
Recombinant Rat Erythropoietin (EPO) (carrier-free) BA
Recombinant Human PCSK9 (carrier-free) BA
Recombinant Human CCL17 (TARC) (ELISA Std.) ELISA
Recombinant Human TIMP-1 (carrier-free) BA
Recombinant Human Arginase I (carrier-free) BA
Recombinant Human MMP-1 (carrier-free) BA
Recombinant Human CD14 (carrier-free) BA
Recombinant Human CTLA-4 (ELISA Std.) ELISA
Recombinant Mouse IL-34 (ELISA Std.) ELISA
Recombinant Human IL-34 (ELISA Std.) ELISA
Recombinant Human Latent TGF-β (ELISA Std.) ELISA
Recombinant Human Mesothelin-Fc Chimera (carrier-free) BA
Recombinant Mouse Thrombopoietin (TPO) (carrier-free) BA
Recombinant Human TIMP-2 (carrier-free) BA
Recombinant Mouse TIMP-1 (carrier-free) BA
Brilliant Violet 421™ Annexin V FC
Alexa Fluor® 488 Streptavidin FC, ICFC
Alexa Fluor® 647 Streptavidin FC, ICFC
Recombinant Mouse CCL3 (MIP-1α) (carrier-free) BA
Recombinant Human LIF (carrier-free) BA
Recombinant Mouse Mesothelin (carrier-free) BA
Recombinant Rat IFN-γ (ELISA Std.) ELISA
Recombinant Mouse IL-17A/F Heterodimer (ELISA Std.) ELISA
Recombinant Human TGF-β1 (ELISA Std.) ELISA
Zombie Violet™ Fixable Viability Kit FC, ICFC, ICC
Zombie Green™ Fixable Viability Kit FC, ICFC, ICC
Zombie Red™ Fixable Viability Kit FC, ICFC, ICC
Zombie UV™ Fixable Viability Kit FC, ICFC
CFSE Cell Division Tracker Kit ICFC, in-vivo Cell Tracking
LEGEND MAX™ Human Adiponectin (Acrp30) ELISA Kit ELISA
Bromodeoxyuridine (BrdU) FC, ICFC
Recombinant Mouse CCL5 (RANTES) (carrier-free) BA
Recombinant Mouse IFN-β (ELISA Std.) ELISA
Recombinant Human IL-9 (carrier-free) BA
Recombinant Mouse Latent TGF-β (ELISA Std.) ELISA
Recombinant Human MMP-3 (carrier-free) BA
APC Streptavidin (High Concentration) FC, ICFC
Recombinant Human CCL2 (MCP-1) (Animal-Free) BA
Recombinant Human VEGFR2-Fc Chimera (carrier-free) BA
Recombinant Human NT-4 (Animal-Free) BA
Cell Activation Cocktail (without Brefeldin A) Activ, FC, ICFC
Recombinant Human RBP4 (carrier-free) BA
Recombinant Rat IFN-γ (carrier-free) BA
Recombinant Human ICOS-Fc Chimera (carrier-free) BA
Cell Activation Cocktail (with Brefeldin A) Activ, FC, ICFC
Recombinant Mouse ICOS-Fc Chimera (carrier-free) BA
Recombinant Mouse VEGF-164 (Animal-Free) BA
Recombinant Human NT-3 (carrier-free) BA
Recombinant Human NT-4 (carrier-free) BA
Recombinant Human FGF-1-acidic (carrier-free) BA
Nunc™ MaxiSorp™ ELISA Plates, Uncoated ELISA
Plate Sealers ELISA
DRAQ7™ FC, ICFC
Recombinant Human WISP-1 (carrier-free) BA
Recombinant Human WNT-7a (carrier-free) BA
Go To Top Version: 1    Revision Date: 07/26/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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