Epitope Tag Big Motif Antibody Sampler Kit

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Other Names
Glutathione-S-transferase, Thioredoxin-1 (Trx1), Fip, FipA, TsnC, TrxA, Thioredoxin Tag, DSRED, Green fluorescent protein, GFP
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A_BigMotifSampler_anti-GST_WB_081817
15 µg of total protein from E. coli lysates with GST expression (Lane 1) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-GST antibody (clone P1A12). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Lane M: MW ladder.
  • A_BigMotifSampler_anti-GST_WB_081817
    15 µg of total protein from E. coli lysates with GST expression (Lane 1) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-GST antibody (clone P1A12). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Lane M: MW ladder.
  • B_BigMotifSampler_anti-GFP_WB_081817
    15 µg each of total protein from 293E (lane2), 293E transfected with GFP fused protein (lane 3), were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-GFP antibody (clone 1GFP63). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder.
  • C_BigMotifSampler_anti-mCherry_WB_081817
    15 µg each of total protein from 293E (lane2), 293E transfected with mCherry fused protein (lane 4), were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-mCherry antibody (clone 8C5.5). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder.
  • D_BigMotifSampler_anti-Thioredoxin1_WB_081817
    25 ng recombinant Thioredoxin-1 protein (Lane 5) was resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-Thioredoxin 1 antibody (clone 4H12A59). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Lane M: MW ladder.
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699902 1 kit 220€
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Description

The Epitope Tag Big Motif Antibody Sampler Kit provides a convenient resource to detect big Epitope Tag fused protein by Western Blot.

GST is commonly used as a tag to create fusion proteins in order to facilitate purification and detection of these proteins of interest. Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems. mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. Thioredoxin is an 11.7 kD E. coli cytoplasmic protein. When used as a fusion partner to produce recombinant protein, thioredoxin has been shown to increase protein solubility and yield, as well as reduce protein aggregation and precipitation. 

For additional antigen details, please refer to individual product datasheets.

 

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD)
Anti-GST P1A12 25 µg GST tagged protein Mouse IgG2b, κ 26
Anti-GFP 1GFP63 25 µg GFP Mouse IgG2a 27
Anti-mCherry 8C5.5 25 µg mCherry Mouse IgG2a 27
Anti-Thioredoxin 1 4H12A59 25 µg E. coli Thioredoxin-1 and thioredoxin tagged recombinant protein Mouse IgG1, κ 12


* For detailed information about each specificity, please follow the provided links. 

Product Details

Formulation
Please refer to individual product datasheets for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Concentration
All antibodies in the kit are at 0.5mg/ml
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-GST: 0.05-0.5 µg/ml
Anti-GFP: 0.05-0.5 µg/ml
Anti-mCherry: 0.1-1 µg/ml
Anti-Thioredoxin 1: 0.05-0.5 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Application References

(PubMed link indicates BioLegend citation)
  1. Nicol C, et al. 2011. Biochem. Biophys. Res. Commun. 405:417. PubMed (Anti-GST)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 08/22/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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