ELISA MAX™ Deluxe Set Human CD26 (DPP4)

Pricing & Availability
Regulatory Status
RUO
Other Names
Dipeptidyl peptidase 4, ADCP2, adenosine deaminase complexing protein 2
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ELISA-MAX_Deluxe_Human_CD26-DPP4_092822
  • ELISA-MAX_Deluxe_Human_CD26-DPP4_092822
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449304 5 plates 280€
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Description

CD26, also known as Dipeptidyl peptidase-4 (DPP4), is a serine exopeptidase expressed by many cell types. It can exist as both a membrane protein and an active soluble form. CD26 has important roles in many processes including, but not limited to, cell signaling, immune regulation, and apoptosis. For instance, it can act as a co-stimulatory receptor for T-cells activation. Additionally, it can cleave multiple chemokines and peptide hormones such as glucagon, shortening their activity. Due to its abundance and multifunctional characteristic, CD26 can be a biomarker for multiple diseases such as immune disfunction, obesity, diabetes, and cancer.

BioLegend ELISA MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solutions for Sandwich ELISA assay. A human CD26 (DPP4) specific capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and human CD26 (DPP4) binds to the immobilized capture antibody. Then, a biotinylated anti-human CD26 (DPP4) detection antibody is added, producing an antibody-antigen-antibody “sandwich”. The Avidin-HRP reagent is subsequently added, followed by TMB Substrate, producing a blue color in proportion to the concentration of CD26 present in wells. Then Stop Solution should be added to wells to terminate the reaction. This step changes the reaction color from blue to yellow. The absorbance in wells should be read at 450 nm and 570 nm using a microplate reader.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human CD26 (DPP4) ELISA MAX™ Capture Antibody (200X)
  • Human CD26 (DPP4) ELISA MAX™ Detection Antibody (200X)
  • Human CD26 (DPP4) Standard Lyophilized
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
5.7 pg/mL
Standard Range
31.25 - 2000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS +
    0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid
    solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4,
    0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm
    filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Antigen-presenting cells, Epithelial cells, T cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Gene ID
P27487 View all products for this Gene ID
UniProt
View information about CD26 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 09/28/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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