ELISA MAX™ Deluxe Set Human Angiopoietin-2

Pricing & Availability
Regulatory Status
RUO
Other Names
ANGPT2, ANG2, ANGPT-2, ANG-2, AGPT2, AGPT-2
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Product Citations
publications
/LEGENDMAX_Human-Angiopoietin-2_ELISA_Kit_0712522
  • /LEGENDMAX_Human-Angiopoietin-2_ELISA_Kit_0712522
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440404 5 plates 296€
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Description

Angiopoietin-2 is a member of the Angiopoietin family of vascular growth factors and a glycoprotein involved in angiogenesis and inflammation. It plays a vital role in embryonic and postnatal angiogenesis and is highly abundant in vascular endothelial cells. Angiopoietin-2 along with Angiopoietin-1, are ligands for the receptor tyrosine kinase Tie2. It is also known to work synergistically with cytokines, such as VEGF, to promote tumor progression and associated angiogenesis. Studies have shown that it is a marker for endothelial activation. Due to its important role in inflammatory conditions, this analyte has a great potential for new disease therapies.

BioLegend’s ELISA MAX™ Deluxe Set Human Angiopoietin-2 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA), utilizing a monoclonal capture antibody and biotinylated monoclonal antibody specific for human Angiopoietin-2. This set contains necessary reagents for the accurate quantification of natural and recombinant human Angiopoietin-2 in serum, plasma, urine and other biological fluids. BioLegend’s ELISA MAX™ Deluxe Sets are cost-effective, sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Angiopoietin-2 ELISA MAX™ Capture Antibody (200X)
  • Human Angiopoietin-2 ELISA MAX™ Detection Antibody (200X)
  • Human Angiopoietin-2 Standard
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Blocking Buffer A (2X)
  • Avidin-HRP (1000X)
  • Substrate Solution F

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
74.40 ± 2.54 pg/mL
Standard Range
125 - 8000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Endothelial cells
Biology Area
Angiogenesis
Gene ID
285 View all products for this Gene ID
UniProt
View information about Angiopoietin-2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 07/25/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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