ELISA MAX™ Deluxe Set Human Angiopoietin-2

Pricing & Availability
Regulatory Status
Other Names
Ave. Rating
Submit a Review
Product Citations
  • LEGENDMAX_Human-Angiopoietin-2_ELISA_Kit_0712522
Cat # Size Price Quantity Check Availability Save
440404 5 plates 296€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Angiopoietin-2 is a member of the Angiopoietin family of vascular growth factors and a glycoprotein involved in angiogenesis and inflammation. It plays a vital role in embryonic and postnatal angiogenesis and is highly abundant in vascular endothelial cells. Angiopoietin-2 along with Angiopoietin-1, are ligands for the receptor tyrosine kinase Tie2. It is also known to work synergistically with cytokines, such as VEGF, to promote tumor progression and associated angiogenesis. Studies have shown that it is a marker for endothelial activation. Due to its important role in inflammatory conditions, this analyte has a great potential for new disease therapies.

BioLegend’s ELISA MAX™ Deluxe Set Human Angiopoietin-2 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA), utilizing a monoclonal capture antibody and biotinylated monoclonal antibody specific for human Angiopoietin-2. This kit is specifically designed for the accurate quantitation of human angiopoietin-2 in serum, plasma, cell culture supernatant, and saliva. BioLegend’s ELISA MAX™ Deluxe Sets are cost-effective, sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Angiopoietin-2 ELISA MAX™ Capture Antibody (200X)
  • Human Angiopoietin-2 ELISA MAX™ Detection Antibody (200X)
  • Human Angiopoietin-2 Standard
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Blocking Buffer A (2X)
  • Avidin-HRP (1000X)
  • Substrate Solution F

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
74.40 ± 2.54 pg/mL
Standard Range
125 - 8000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Endothelial cells
Biology Area
Gene ID
285 View all products for this Gene ID
View information about Angiopoietin-2 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 03/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account