Direct-Blot™ HRP anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
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Product Citations
publications
L5_DirectBlotHRP_DYKDDDDK_WB_Image_010616
50 ng of recombinant GST protein tagged with the DYKDDDDK peptide was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-DYKDDDDK tag (clone L5). Proteins were visualized using chemiluminescence detection.
  • L5_DirectBlotHRP_DYKDDDDK_WB_Image_010616
    50 ng of recombinant GST protein tagged with the DYKDDDDK peptide was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-DYKDDDDK tag (clone L5). Proteins were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
637312 25 µl 68€
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637311 100 µl 232€
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Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
DYKDDDDK tag epitope
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Uche UU, et al. 2018. J Exp Med. 215:3165. PubMed
Publication Library
RRID
AB_2734514 (BioLegend Cat. No. 637312)
AB_2566706 (BioLegend Cat. No. 637311)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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