Cell Staining Buffer

Pricing & Availability
Other Names
Staining Buffer, Washing Buffer
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420201 500 ml 60€
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Description

Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce non-specific binding of antibodies and fluorochrome reagents to target cells. It also contains a metabolic inhibitor, sodium azide (NaN3), to inhibit patching and capping of cell surface antigens. To prevent interference in biotin/avidin indirect staining protocols, Cell Staining Buffer is formulated without biotin.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ICC, ICFC - Reported in the literature

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
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  2. Garcia J, et al. 2013. J Immunol. 191:1063. PubMed
  3. Lukas S, et al. 2013. J Biomol Screen. 19:407. PubMed
  4. Murali V, et al. 2014. J Virol. 88:903. PubMed
  5. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  6. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  7. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
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  12. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  13. Pino P, Cardona A 2011. J Vis Exp. 48: 2348. PubMed
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Publication Library

Antigen Details

Cell Type
Tregs
Gene ID
NA

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Go To Top Version: 1    Revision Date: 11/30/2012

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