Biotin anti-mouse PCSK9 Antibody

Pricing & Availability
Poly5251 (See other available formats)
Regulatory Status
Other Names
Neural Apoptosis Regulated Convertase 1 (NARC1), proprotein convertase PC9, subtilisin/kexin-like protease PC9, FH3, HCHOLA3, AI415265, AI747682
Goat Polyclonal Ig
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525104 50 µg 300€
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Proprotein convertase subtilisin/kexin 9 (PCSK9) possesses a signal peptide, a prosegment, a catalytic domain, a hinge region, and a C-terminal Cys-His-rich domain. This protein is primarily synthesized and secreted by hepatocytes. Besides the liver, it is also expressed in the small intestine, kidney, and brain. It is also present in plasma. Upon translocation to the endoplasmic reticulum, the prosegment of PCSK9 is autocatalytically cleaved and secreted as a stable, enzymatically inactive, non-covalent complex. Elevated low density lipoprotein-cholesterol (LDLc) level is a major risk factor for cardiovascular disease and atherosclerosis. PCSK9 is a regulator of LDLc levels through the binding of LDLR, which subsequently leads to the degradation of LDLR. PCSK9 null mice fed with a high fat diet demonstrated a four-fold decrease in cholesterol accumulation, which stresses the important role played by PCSK9 in the development of cardiovascular diseases. PCSK9 also binds to other LDLR family members such as very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER), and apolipoprotein receptor 2 (LRP8/APOER2), which leads to their degradation in intracellular acidic compartments. Like the LDLR, gene expression of PCSK9 is positively regulated by sterol regulatory element-binding protein 2 (SREBP-2), which is a transcription factor that is activated in response to cellular cholesterol depletion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant mouse PCSK9
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of PCSK9 recombinant protein ranging from 4000 to 62.5 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated Poly5251 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M114G10 (Cat. No. 532402) antibody as the capture antibody.


Note: For testing mouse PCSK9 in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443207 & 443208) are specially developed and recommended.

AB_2686956 (BioLegend Cat. No. 525104)

Antigen Details

Monomer. The molecular mass of a full-length and mature protein is 74.8 kD and 53.7 kD, respectively.

Hepatocytes, renal cells, neuroepithelioma, and Schwann cells.

PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and therefore, LDLc levels. PCSK9 is regulated by SREBP-2.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators
Antigen References

1. Seidah NG, et al. 2003. Proc. Natl. Acad. Sci. USA 100:928.
2. Naureckiene S, et al. 2003. Arch. Biochem. Biophys. 420:55.
3. Costet P, et al. 2008. Trends Biochem. Sci. 33:426.
4. Grefhorst A, et. al. 2008. J. Lipid Res. 49:1303.
5. Tavori H, et al. 2013. Circulation 127:2403.
6. Denis M, et al. 2012. Circulation 125:894.
7. Tibolla G, et al. 2011. Nutr. Metab. Cardiovasc. Dis. 21:835.

Gene ID
100102 View all products for this Gene ID
View information about PCSK9 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 03/30/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Biotin anti-mouse PCSK9


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