Biotin anti-mouse NGAL (Lipocalin-2) Antibody

Pricing & Availability
Clone
M0417A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
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532304 100 µg 180,00€
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Description

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. Mouse NGAL is detected in lung, spleen, uterus, vagina, and epididymis. It is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising new markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.1 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The biotinylated M0417A8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M047A10 (Cat. No. 532202) antibody as the capture antibody.

Note: For testing mouse NGAL in cell culture supernatant, serum, plasma, urine and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443707 & 443708) are specially developed and recommended.

Product Citations
RRID
AB_2686952 (BioLegend Cat. No. 532304)

Antigen Details

Structure
195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9.
Ligand/Receptor
SLC22A17 (24p3R)
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209. 
2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663. 
3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832. 
4. Flo TH, et al. 2004. Nature 432:917. 
5. Goetz DH, et al. 2002. Mol. Cell. 10:1033. 
6. Leung L, et al. 2012. PLoS One 7:e46677. 
7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.

Gene ID
16819 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 10/29/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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