- Poly5186 (See other available formats)
- Regulatory Status
- Other Names
- Lactadherin, Milk fat globule-EGF factor 8, MFG-E8, MFGE-8, MFGE8 MFGM, Sperm surface protein SP47, MP47 PAS-6/7, bovine-associated mucoprotein, BA-46, P47
- Goat Polyclonal Ig
- Ave. Rating
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- Product Citations
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Lactadherin, a novel adhesion molecule, also known as MFG-E8, is a widely distributed glycoprotein (~50 kD) that has been found in macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinioma cells, and also in breast milk and around blood vessels. Lactadherin contains an N-terminal EGF-like motif with an inserted RGD cell adhesion sequence; it also contains a C-terminal sequence sharing homology with the discoidin family of lectin domains, including the phospholipid-binding domains of blood clotting factors V and VIII. Lactadherin promotes RGD-dependent cell attachment of a variety of cell types, but most notably of cells that express the integrin heterodimer, alpha vβ3. The C1/C2 domain binds specifically to phosphatidylserine. Mounting evidence has shown that Lactadherin plays a far more important role than just protecting the newborn, a function originally identified. Lactadherin is anticoagulant by blocking phosphatidylserine-containing membrane sites for blood coagulation proteins. It is involved in cell death by its ability to recognize specifically phosphatidylserine exposed on apoptotic cells. Lactadherin is also crucial in VEGF-dependent neovascularization and has been identified as an important target for the modulation of neovascularization. In addition, Lactadherin was identified as a novel target for radioimmunotherapy in breast cancer, and is also important in the initiation and/or progression of Alzheimer's disease.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- 293E cells expressed recombinant mouse Lactadherin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25 - 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse Lactadherin recombinant protein ranging from 6000 to 94 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA: The purified poly5186 (Cat. No. 518602) antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5186 antibody (Cat. No. 518603) as the detection antibody.
Note: For testing mouse lactadherin in serum, plasma or cell culture supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 438107 & 438108) are specially developed and recommended.
AB_2562986 (BioLegend Cat. No. 518603)
- Glycoprotein, approx. 50 kD
Macrophages, dendritic cells, vascular smooth muscle cells, astrocytes, microglia, breast carcinoma cells
- Anticoagulant, apoptosis, and neovascularization; also important in the initiation and/or progression of Alzheimer's disease
- Integrin, phosphatidylethanolamine, phosphatidylserine
- alpha v/β3 and alpha v/β5 receptors
- Cell Type
- Astrocytes, Dendritic cells, Macrophages, Microglia
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Gene ID
- 17304 View all products for this Gene ID
- View information about Lactadherin on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
|Biotin anti-mouse Lactadherin||Poly5186||ELISA Detection|
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