Biotin anti-mouse IL-27 Antibody

Pricing & Availability
B02P6E6 (See other available formats)
Regulatory Status
Other Names
Interleukin-27 p28, IL-27-A, IL-27 subunit alpha
Rat IgG2a, κ
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Product Citations
Cat # Size Price Quantity Check Availability Save
525903 50 µg 118€
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525904 500 µg 334€
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IL-27, a heterodimer protein, belongs to the IL-12 cytokine family. IL-27 is composed of the Epstein-Barr virus-induced gene 3 (EBI-3), a 34 kD glycoprotein that is related to the p40 subunit of IL-12; and p28, a 28 kD glycoprotein that is related to the p35 subunit of IL-12 and p19 subunit of IL-23.

IL-27 triggers expansion of antigen-specific naïve CD4+ T cells, promotes polarization of Th1 cells, and inhibits the development of Th2 and Th17 cells. It is known that IL-27 is an early product of activated antigen-presenting cells (APCs). IL-27 plays an important role in regulating the activity of B and T lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant mouse IL-27
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.125 µg/ml of the capture antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Detection: To measure mouse IL-27, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified MM27-7B1 antibody (Cat. No. 516902) as the capture antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.
 For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended.

AB_2563423 (BioLegend Cat. No. 525903)
AB_2563424 (BioLegend Cat. No. 525904)

Antigen Details

A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Produced by antigen-presenting cells, including macrophages and dendritic cells.
Drives rapid clonal expansion of naïve CD4+ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
T cells and NK cells.
IL-27R (TCCR) (WSX-1).
Cell Type
Antigen-presenting cells, Macrophages, Dendritic cells, B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family
Antigen References

1. Salcedo R, et al. 2004. J. Immunol. 173:7170.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Pflanz S, et al. 2002. Immunity 16:779.
4. Diveu C, et al. 2009. J. Immunol. 182:5748.
5. Morishima N, et al. 2005. J. Immunol. 175:1686.
6. Liu J, et al. 2007. J. Exp. Med. 204:141.

Gene ID
246779 View all products for this Gene ID
View information about IL-27 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 06/12/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Biotin anti-mouse IL-27


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