Loading...

Biotin anti-mouse CX3CR1 Antibody

Pricing & Availability
Clone
SA011F11 (See other available formats)
Regulatory Status
RUO
Other Names
Chemokine (C-X3-C motif) receptor 1, Fractalkine receptor, GPR13, CCRL1, CMKBRL1, CMKDR1, V28
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
SA011F11_Biotin_CX3CR1_Antibody_FC_1_051315
C57BL/6 mouse splenocytes were stained with CD11b APC and biotinylated CX3CR1 (clone SA011F11) (top) or biotinylated mouse IgG2a, κ isotype control (bottom), followed by SAV-PE.
  • SA011F11_Biotin_CX3CR1_Antibody_FC_1_051315
    C57BL/6 mouse splenocytes were stained with CD11b APC and biotinylated CX3CR1 (clone SA011F11) (top) or biotinylated mouse IgG2a, κ isotype control (bottom), followed by SAV-PE.
  • SA011F11_Biotin_CX3CR1_Antibody_FC_2_051315
Compare all formats
Cat # Size Price Quantity Check Availability Save
149018 100 µg 221€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CX3CR1 is a 40 kD, G-protein coupled receptor, with seven transmembrane regions. CX3CR1 is expressed by resident and alternatively activated macrophages (M2), a subset of monocytes, dendritic cells (DCs), NK cells, a subset of memory T cells, and mast cells. CX3CR1 is involved in cell recruitment during inflammation and participates in cell adhesion and extravasation from blood vessels. Its ligand is CX3CL1, also known as fractalkine or neurotactin. CX3CR1 is also a coreceptor for HIV1 and variations in this gene leads to increased susceptibility to HIV. In the brain, it is expressed by glial cells, which interact with CX3CL1 expressed by neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse CX3CR1-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide; may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Cell Separation (MojoSort™) - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. For magnetic cell separation with MojoSort™ streptavidin Nanobeads (Cat. No. 480015/480016), the suggested use of this reagent is ≤0.15 µg per 10 million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

View more applications data for a different format of this clone in our Scientific Poster Library.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Product Citations
  1. Anthony SM, et al. 2021. eLife. 10:00. PubMed
  2. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  3. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  4. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  5. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
RRID
AB_2565701 (BioLegend Cat. No. 149018)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions, 40 kD.
Distribution

Resident and alternatively activated macrophages (M2), subset of monocytes, dendritic cells (DCs), natural killer cells (NK), subset of memory T cells, mast cells, glial cells, and astrocytes.

Function
Involved in cell recruitment during inflammation, participates in cell adhesion and extravasation from blood vessels, coreceptor for HIV1.
Ligand/Receptor
CX3CL1 (fractalkine, neurotactin).
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Microglia, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ponzetta A, et al. 2013. J. Immunol. 191:5684.
2. Jacquelin S, et al. 2013. Blood. 122:674.
3. Garcia JA, et al. 2013. J. Immunol. 191:1063.
4. Lee YS, et al. 2013. Cell. 153:413.
5. Shechter R, et al. 2013. Immunity. 38:555.

Gene ID
13051 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 05/05/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CX3CR1

  • Alexa Fluor® 647 anti-mouse CX3CR1

  • PE anti-mouse CX3CR1

  • APC anti-mouse CX3CR1

  • PerCP/Cyanine5.5 anti-mouse CX3CR1

  • Brilliant Violet 421™ anti-mouse CX3CR1

  • Brilliant Violet 510™ anti-mouse CX3CR1

  • FITC anti-mouse CX3CR1

  • Alexa Fluor® 488 anti-mouse CX3CR1

  • PE/Dazzle™ 594 anti-mouse CX3CR1

  • PE/Cyanine7 anti-mouse CX3CR1

  • Biotin anti-mouse CX3CR1

  • Brilliant Violet 605™ anti-mouse CX3CR1

  • Brilliant Violet 785™ anti-mouse CX3CR1

  • Brilliant Violet 711™ anti-mouse CX3CR1

  • Brilliant Violet 650™ anti-mouse CX3CR1

  • Alexa Fluor® 700 anti-mouse CX3CR1

  • APC/Fire™ 750 anti-mouse CX3CR1

  • Pacific Blue™ anti-mouse CX3CR1

  • TotalSeq™-A0563 anti-mouse CX3CR1

  • TotalSeq™-C0563 anti-mouse CX3CR1

  • TotalSeq™-B0563 anti-mouse CX3CR1

  • APC/Cyanine7 anti-mouse CX3CR1 Antibody

  • PE/Cyanine5 anti-mouse CX3CR1

  • PE/Fire™ 700 anti-mouse CX3CR1

  • APC/Fire™ 810 anti-mouse CX3CR1

  • PE/Fire™ 640 anti-mouse CX3CR1

  • PE/Fire™ 810 anti-mouse CX3CR1
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account