Biotin anti-human NGAL (Lipocalin-2) Antibody

Pricing & Availability
Clone
M049D6 (See other available formats)
Regulatory Status
RUO
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
503304 500 µg 320,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. NGAL is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.15 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 2500 to 39.1 pg/mL is recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated M049D6 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0415B7 (Cat. No. 661902) antibody as the capture antibody.

 

Note: For testing human NGAL in cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443407 & 443408) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.
  2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
  3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
  4. Flo TH, et al. 2004. Nature 432:917
  5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
  6. Leung L, et al. 2012. PLoS One 7:e46677
  7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.
RRID
AB_2650710 (BioLegend Cat. No. 503304)

Antigen Details

Structure
193 amino acid monomer with a predicted molecular weight of approximately 22 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils. Increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9
Ligand/Receptor
SLC22A17 (24p3R)
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Gene ID
3934 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 04/04/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human NGAL (Lipocalin-2)

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account