Biotin anti-human IL-22 Antibody

Pricing & Availability
Poly5161 (See other available formats)
Regulatory Status
Other Names
TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
Goat Polyclonal Ig
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Product Citations
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516103 50 µg 300€
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IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor).   IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29.  Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1).  Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human IL-22, amino acids Ala34-Ile179 (Accession# NM_020525) was expressed in E. coli.
Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 8000 to 125 pg/ml are recommended.

Application Notes

ELISA Detection: The biotinylated Poly5161 antibody is useful as a detection antibody in a sandwich ELISA assay, when used in conjunction with purified poly5161 (Cat. No. 516101) antibody as the capture antibody and recombinant human IL-22 (Cat. No. 571309) as the standard.

AB_2561412 (BioLegend Cat. No. 516103)

Antigen Details

Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells, Th17
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
IL-22R1 and IL-10R2 receptor chains
In sandwich ELISA, less than 10% cross-reactivity with recombinant mouse IL-22.
Cell Type
T cells, Macrophages, Th17, Mast cells
Biology Area
Molecular Family
Antigen References

1. Nagalakshmi ML, et al. 2004. Intern. Immunopharmacol. 4:679.
2. Kebir H, et al. 2007. Nat. Med. 13:1173.
3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807.
4. Pene J, et al. 2008. J. Immunol. 180:7423.
5. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
6. Xie MH, et al. 2000. J. Biol. Chem. 265:31335.
7. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
8. Chung Y, et al. 2006. Cell Res. 16:902.
9. Trifari S, et al. 2009. Nat. Immunol. 10:864.

Gene ID
50616 View all products for this Gene ID
View information about IL-22 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IL-22 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IL-22 Poly5161 ELISA Capture
Biotin anti-human IL-22 Poly5161 ELISA Detection
Go To Top Version: 2    Revision Date: 01/07/2013

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human IL-22

  • Biotin anti-human IL-22


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