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Biotin anti-human CD106 Antibody

Pricing & Availability
Clone
STA (See other available formats)
Regulatory Status
RUO
Workshop
V A013
Other Names
VCAM-1, INCAM-110
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
STA_Biotin_070507
TNF-α stimulated HUVEC cells stained with biotinylated STA, followed by Sav-PE
  • STA_Biotin_070507
    TNF-α stimulated HUVEC cells stained with biotinylated STA, followed by Sav-PE
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305804 100 µg 151€
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Description

CD106 is a 110 kD single chain type I glycoprotein also known as VCAM-1 and INCAM-110. It is expressed predominantly on activated vascular endothelium but has also been identified on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. Expression on endothelial cells as well as many other cells is induced by inflammatory stimuli and cytokines. Activated endothelial cells can release soluble forms of CD106 which can be detected in the blood. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 that contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation2, ELISA2 capture for sCD106, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Leca G, et al. 1995. J. Immunol. 154:1069. (ELISA IP)
  3. Yen YT, et al. 2006. J. Virol. 80:2648. (IF) PubMed
  4. Dmitrieva NI, et al. 2015. PloS One.10:128870. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_314560 (BioLegend Cat. No. 305804)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 110 kD
Distribution

Activated endothelial cells, endothelial progenitors, follicular dendritic cells

Function
Leukocyte adhesion, transmigration, costimulation
Ligand/Receptor
VLA-4 (CD49d/CD29)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Carlos T, et al. 1994. Blood 84:2068.
2. Jones E, et al. 1995. Nature 373:539.

Gene ID
7412 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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