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Biotin anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# BIOT-101L)

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# BIOT-101L
Isotype
Mouse IgG1, κ
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Product Citations
publications
16B12_Biotin_HA.11_1_011918
Total lysates (15 µg protein) from CHO (lane 1) and CHO HA stable cells (lane 2) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:10000 (0.1 µg/ml) Biotin-HA antibody, clone 16B12. Proteins were visualized using chemiluminescence detection by incubation with HRP Avidin secondary antibody (Cat. No. 405103, 1:10000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 16B12_Biotin_HA.11_1_011918
    Total lysates (15 µg protein) from CHO (lane 1) and CHO HA stable cells (lane 2) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:10000 (0.1 µg/ml) Biotin-HA antibody, clone 16B12. Proteins were visualized using chemiluminescence detection by incubation with HRP Avidin secondary antibody (Cat. No. 405103, 1:10000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 16B12_Biotin_HA.11_1_011918
    Immunofluorescence of HA-tagged plasmid-transfected A549 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Biotin-HA Mouse primary antibody (Clone 16B12). Alexa Fluor® 594 (Red) Streptavidin (Cat. No. 405240) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, (B, D) is 1/300 and (C) is 1/500. Concentrations for (A-C) is 1 μg/ml, (D) is 0.5 µg/ml.
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901505 100 µL 336€
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution + 50% Glycerol + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
FC, ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 µg per mL (1:10000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL (1:1000-1:2000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
  1. Gregory JA, et al. 2020. Cells. 9:00. PubMed
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  3. Lambert AR, et al. 2020. Structure. 28(7):760-775. PubMed
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RRID
AB_2565023 (BioLegend Cat. No. 901505)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 7    Revision Date: 12/03/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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