- SK1 (See other available formats)
- Regulatory Status
- Other Names
- T8, Leu2
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.Product Details
- Human, Cross-Reactivity: African Green, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.
(PubMed link indicates BioLegend citation)
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
- Campanelli R, et al. 2002. Intl. Immunol. 14:39.
- Evans RL, et al. 1981. Immunol. 78:544.
- Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
- Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
- Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
- Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
AB_2860889 (BioLegend Cat. No. 344763)
AB_2860890 (BioLegend Cat. No. 344764)
- Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Majority of thymocytes, T cell subset, NK cells
- MHC class I co-receptor, thymic differentiation, T cell activation
- MHC Class I molecules
- Cell Type
- NK cells, T cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
- Gene ID
- 925 View all products for this Gene ID
- View information about CD8 on UniProt.org
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.