APC/Fire 750 anti-rat CD90/mouse CD90.1 Thy-1.1 Antibody anti-CD90.1

Pricing & Availability

Clone: OX-7 (See other available formats)
Regulatory Status: RUO
Other Names: Thy-1, Thy-1.1
Isotype: Mouse IgG1, κ
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Product Citations: publications

OX-7_APCFire750_CD90dot1_Antibody_FC_021417 — Lewis rat thymocytes were stained with APC/Fire™ 750 anti-rat CD90/mouse CD90.1 (filled histogram) or APC/Fire™ 750 mouse IgG1, κ isotype control (open histogram).

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Size

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202543

25 µg

100€

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202544

100 µg

272€

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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Rat, Mouse

Reported Reactivity

Rabbit, Guinea Pig

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Rat thymocyte Thy-1 antigen

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical⁷ and immunofluorescent⁸ staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation¹, Western blotting¹, in vitro activation of leukocytes², induction of endothelial cell permeability³, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo⁴.

Application References (PubMed link indicates BioLegend citation)

Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
Nakashima I, et al. 1991. J. Immunol. 147:1153.
Ishizu A, et al. 1995. Int. Immunol. 7:1939.
Eitner F. 1997. Kidney. Int. 51:69.
Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
Daniel C, et al. 2012. Lab Invest. 92:812. (IHC-P)
Li B, et al. 2006. Kidney Int. 69:323. (ICC)
Uchimura H, et al. 2005. J Am Soc Nephrol. 16(4):997-1004. (IHC-F)
Inagi R, et al. 2008. J Am Soc Nephrol. 19(5):915-22. (IHC-P)

RRID

AB_2650816 (BioLegend Cat. No. 202543)
AB_2650817 (BioLegend Cat. No. 202544)

Antigen Details

Structure: Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
Function: Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: Interacts with CD45
Cell Type: B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
Gene ID: 21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt: View information about CD90/CD90.1 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD90/CD90.1 Reagents Request Custom Conjugation

Description	Clone	Applications
PE/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Purified anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC,IHC-F,ICC,IHC-P,IP,WB
FITC anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Alexa Fluor® 488 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC,IHC-F
Alexa Fluor® 647 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC,IHC-F
PerCP/Cyanine5.5 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
APC/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
PerCP anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
PE anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
APC anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Biotin anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Brilliant Violet 650™ anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Brilliant Violet 510™ anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Brilliant Violet 605™ anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Brilliant Violet 711™ anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
PE/Dazzle™ 594 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
APC/Fire™ 750 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	FC
Alexa Fluor® 594 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	IHC-F
TotalSeq™-A0380 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	PG
TotalSeq™-B0380 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	PG
TotalSeq™-C0380 anti-rat CD90/mouse CD90.1 (Thy-1.1)	OX-7	PG

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 12/15/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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