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APC/Fire™ 750 anti-mouse CD21/CD35 (CR2/CR1) Antibody

Pricing & Availability
Clone
7E9 (See other available formats)
Regulatory Status
RUO
Other Names
CR2/CR1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7E9_APCFire750_CD21-CD35_Antibody_050720.png
C57BL/6 splenocytes were stained with CD45R/B220 PE and CD21/CD35 (CR2/CR1) (clone 7E9) APC/Fire™ 750 (left) or rat IgG2a, κ isotype control APC/Fire™ 750 (right).
  • 7E9_APCFire750_CD21-CD35_Antibody_050720.png
    C57BL/6 splenocytes were stained with CD45R/B220 PE and CD21/CD35 (CR2/CR1) (clone 7E9) APC/Fire™ 750 (left) or rat IgG2a, κ isotype control APC/Fire™ 750 (right).
Compare all formats See APC/Fire™ 750 spectral data
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123433 25 µg 113€
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123434 100 µg 306€
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Description

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD35/CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Boackle S, et al. 2001 Immunity 15:775.
  2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
  3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
  4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
RRID
AB_2860651 (BioLegend Cat. No. 123433)
AB_2876439 (BioLegend Cat. No. 123434)

Antigen Details

Structure
145 kD/190 kD type I transmembrane glycoprotein
Distribution

B cells, follicular dendritic cells

Function
Signal transduction
Ligand/Receptor
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.

Gene ID
12902 View all products for this Gene ID 12946 View all products for this Gene ID
UniProt
View information about CD21/CD35 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/07/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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