APC/Cyanine7 anti-mouse-rat XCR1 Antibody, XCR1, ZET

Pricing & Availability

Clone: ZET (See other available formats)
Regulatory Status: RUO
Other Names: GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype: Mouse IgG2b, κ
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Product Citations: publications

ZET_APCCyanine7_XCR1_Antibody_102918 — Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c FITC, CD8a PE, and XCR1 (clone ZET) APC/Cyanine7 (left) or mouse IgG2b, ? APC/Cyanine7 isotype control (right). Data shown was gated on the CD11c⁺ population.

Compare all formats See APC/Cyanine7 spectral data

Cat #

Size

Price

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148223

25 µg

137€

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148224

100 µg

315€

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Description

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α⁺ conventional dendritic cells capable of antigen cross-presentation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues².

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)

Product Citations

Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed

RRID

AB_2783117 (BioLegend Cat. No. 148223)
AB_2783118 (BioLegend Cat. No. 148224)

Antigen Details

Structure: Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution: Subset of CD8α⁺ conventional dendritic cells.
Function: Mediates chemotaxis to XCL1, defines a population of CD8α⁺ dendritic cells capable of antigen cross-presentation to CD8⁺ T cells.
Ligand/Receptor: XCL1 and XCL2.
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.
Gene ID: 23832 View all products for this Gene ID 301086 View all products for this Gene ID
UniProt: View information about XCR1 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Chemokine Receptor Biology

Related FAQs

There are no FAQs for this product.

Other Formats

View All XCR1 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse/rat XCR1	ZET	FC
Purified anti-mouse/rat XCR1	ZET	FC,IHC-F
APC anti-mouse/rat XCR1	ZET	FC
PerCP/Cyanine5.5 anti-mouse/rat XCR1	ZET	FC
FITC anti-mouse/rat XCR1	ZET	FC
Biotin anti-mouse/rat XCR1	ZET	FC
Alexa Fluor® 647 anti-mouse/rat XCR1	ZET	FC
Brilliant Violet 421™ anti-mouse/rat XCR1	ZET	FC
Brilliant Violet 510™ anti-mouse/rat XCR1	ZET	FC
Brilliant Violet 650™ anti-mouse/rat XCR1	ZET	FC
APC/Cyanine7 anti-mouse/rat XCR1	ZET	FC
Brilliant Violet 785™ anti-mouse/rat XCR1	ZET	FC
TotalSeq™-A0568 anti-mouse/rat XCR1	ZET	PG
TotalSeq™-C0568 anti-mouse/rat XCR1	ZET	PG
TotalSeq™-B0568 anti-mouse/rat XCR1 Antibody	ZET	PG
PE/Dazzle™ 594 anti-mouse/rat XCR1	ZET	FC
Brilliant Violet 605™ anti-mouse/rat XCR1	ZET	FC
Spark UV™ 387 anti-mouse/rat XCR1	ZET	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/29/2018

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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