Loading...

APC/Cyanine7 anti-mouse CD45RB Antibody

Pricing & Availability
Clone
C363-16A (See other available formats)
Regulatory Status
RUO
Other Names
CD45RB
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
C363-16A_APCCyanine7_CD45RB_Antibody_022519
C57BL/6 splenocytes were stained with CD4 FITC and CD45RB (clone C363-16A) APC/Cyanine7 (left), or rat IgG2a, ? APC/Cyanine isotype control (right).
  • C363-16A_APCCyanine7_CD45RB_Antibody_022519
    C57BL/6 splenocytes were stained with CD4 FITC and CD45RB (clone C363-16A) APC/Cyanine7 (left), or rat IgG2a, ? APC/Cyanine isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103310 100 µg 184€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cloned mouse Th2 cell lines
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Bottomly K, et al. 1989. Eur. J. Immunol. 19:617.
  2. Norian LA and Allen PM. 2004. J. Immunol. 173:835.
Product Citations
  1. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  2. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
RRID
AB_528821 (BioLegend Cat. No. 103310)

Antigen Details

Structure
Phosphatase receptor (CD45 gene exon 5), 220 kD
Distribution

Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells

Function
Phosphatase, T cell development and activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, Thy1
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD45RB

  • PE anti-mouse CD45RB

  • Purified anti-mouse CD45RB

  • APC/Cyanine7 anti-mouse CD45RB

  • Alexa Fluor® 647 anti-mouse CD45RB

  • PerCP/Cyanine5.5 anti-mouse CD45RB

  • Pacific Blue™ anti-mouse CD45RB

  • PE/Cyanine7 anti-mouse CD45RB

  • APC anti-mouse CD45RB

  • TotalSeq™-C1063 anti-mouse CD45RB Antibody

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account