- BNI3 (See other available formats)
- Regulatory Status
- Other Names
- CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
- Mouse IgG2a, κ
- Ave. Rating
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- Product Citations
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CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.
- Verified Reactivity
- Antibody Type
- Host Species
- Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.
(PubMed link indicates BioLegend citation)
- Linsley PS, et al. 1992. J. Exp. Med. 176:1595.
- Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.
AB_2892451 (BioLegend Cat. No. 369633)
AB_2892451 (BioLegend Cat. No. 369634)
- Ig superfamily and 33 kD.
Activated T cells and B cells.
- Negative regulator of T cell activation.
- Antigen presenting cells, such as dendritic cells.
- B7-1 (CD80), B7-2 (CD86).
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.
- Gene ID
- 1493 View all products for this Gene ID
- View information about CD152 on UniProt.org
Related Pages & Pathways
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD152 (CTLA-4)
PE anti-human CD152 (CTLA-4)
Brilliant Violet 421™ anti-human CD152 (CTLA-4)
Brilliant Violet 605™ anti-human CD152 (CTLA-4)
PerCP/Cyanine5.5 anti-human CD152 (CTLA-4)
Biotin anti-human CD152 (CTLA-4)
APC anti-human CD152 (CTLA-4)
PE/Cyanine7 anti-human CD152 (CTLA-4)
PE/Dazzle™ 594 anti-human CD152 (CTLA-4)
TotalSeq™-A0151 anti-human CD152 (CTLA-4)
TotalSeq™-C0151 anti-human CD152 (CTLA-4)
Brilliant Violet 785™ anti-human CD152 (CTLA-4)
TotalSeq™-B0151 anti-human CD152 (CTLA-4)
APC/Fire™ 750 anti-human CD152 (CTLA-4)
Alexa Fluor® 647 anti-human CD152 (CTLA-4)
APC/Cyanine7 anti-human CD152 (CTLA-4) Antibody
Brilliant Violet™ 711 anti-human CD152 (CTLA-4)
TotalSeq™-D0151 anti-human CD152 (CTLA-4)
PE/Fire™ 640 anti-human CD152 (CTLA-4)
Spark Red™ 718 anti-human CD152 (CTLA-4)