- 2G12 (See other available formats)
- Regulatory Status
- Other Names
- CC CKR-4, CHEMR1, Cmkbr4, LESTR, MGC151418, Sdf1r
- Armenian Hamster IgG
- Ave. Rating
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- Product Citations
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Mouse CCR4 cDNA contains 1531 bp, and encodes a protein of 360 amino acids that is 85% identical to human CCR4. CCR4 binds CCL17 (TARG) and CCL22 (MDC). Naïve T cells, bearing receptors for cutaneous antigens, become activated in skin-draining lymph nodes and express cutaneous lymphocyte antigen (CLA), which confers to these cells the capacity to migrate into the skin to exert their normal effector functions (1). CCR4 and CCR10 play an important role in the ligand-mediated recruitment of T cells into the skin in mice and humans, specifically with regards to tethering, firm adhesion, and subsequent extravasation to the site of injury (2,3). CCR4 is expressed in cutaneous regulatory T cells (Tregs). These cells are crucial for the induction and maintenance of self-tolerance and are present in peripheral tissues such as skin and gut under normal, noninflamed conditions (4).In addition, recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor and its ligand CCL22 (5).Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume.
- Excitation Laser
Red Laser (633 nm)
(PubMed link indicates BioLegend citation)
- Saito K, et al. 2008. J. Immunol. 181:6889. PubMed
- Ueha S, et al. 2007. J. Leukoc. Biol. 82:1230. PubMed
- 3. Sharma R, et al. 2009 J. Immunol. 183:1065 (FC) PubMed
- Dogan R, et al. 2011. J. Leukoc. Biol. 89:93. PubMed
- Liu H, et al. 2011. Sci Transl Med. 3:82. PubMed.
- Shankar SP, et al. 2012. J Immunol. 188:6347. PubMed.
- Product Citations
AB_1279135 (BioLegend Cat. No. 131211)
AB_2074507 (BioLegend Cat. No. 131212)
- GPCR, chemokine receptor, seven transmembrane receptor
Memory T cells, macrophages, platelets, basophils, Th2 cells, and Tregs
- CCR4 and its ligands (CCL17 and CCL22) are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.
- Interaction between CCR4 and its ligand TARC/CCL17 on activated endothelial cells mediates T cell extravasation by stimulating integrin-dependent adhesion
- CCl17 and CCL22
- Cell Type
- Basophils, Embryonic Stem Cells, Macrophages, Platelets, T cells, Th2, Tregs
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Biederman T, et al. 2002. Eur. J. Immun. 32:3171.
2. Mirshahpanah P, et al. 2008. Exp. Dermatol. 17:30.
3. Kusumoto M, et al. 2007. J. Interferon Cytokine Res 27:901.
4. Clark RA and Kupper TS. 2006. Blood 109:194.
5. Lee I, et al. 2005. J. Exp. Med. 201:1037.
- Gene ID
- 12773 View all products for this Gene ID
- View information about CD194 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
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