APC anti-human CD152 (CTLA-4) Antibody

Pricing & Availability
Clone
BNI3 (See other available formats)
Regulatory Status
RUO
Other Names
CTLA4, Cytotoxic T-Lymphocyte Antigen 4, CTLA-4
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
BNI3_APC_CD152_Antibody_1_040720
Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without brefeldin) for 4 hours, surface stained with CD3 PE, fixed, permeabilized, and intracellularly stained with CD152 (CTLA-4) (clone BNI3) APC (left), or mouse IgG2a, κ APC isotype control (right).
  • BNI3_APC_CD152_Antibody_1_040720
    Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail (without brefeldin) for 4 hours, surface stained with CD3 PE, fixed, permeabilized, and intracellularly stained with CD152 (CTLA-4) (clone BNI3) APC (left), or mouse IgG2a, κ APC isotype control (right).
  • BNI3_APC_CD152_Antibody_2_040720
    PHA-stimulated human peripheral blood mononuclear cells (3 days) were stained with CD3 PE and anti-human CD152 (clone BNI3) APC (left), or mouse IgG2a, κ APC isotype control (right).
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369611 25 tests 88€
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369612 100 tests 212€
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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA-4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA-4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human CTLA-4 and constant regions of the human IgG heavy chain (CTLA-4/IgG)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Based on in-house testing, we do not recommend using clone BNI3 for immunohistochemistry of paraffin-embedded tissue section.

Application References

(PubMed link indicates BioLegend citation)
  1. Linsley PS, et al. 1992. J. Exp. Med. 176:1595.
  2. Bonzheim I, et al. 2008. Am. J. Clin. Pathol. 130:613.
Product Citations
  1. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  2. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  3. Lu T, et al. 2023. Cancer Immunol Immunother. :. PubMed
  4. Lee PS, et al. 2022. MAbs. 14:2024642. PubMed
  5. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  6. Navarrete-Bernal MGC, et al. 2020. Front Oncol. 1.254166667. PubMed
  7. Wang X, et al. 2020. Exp Ther Med. 19:3698. PubMed
  8. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  9. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID
AB_2632873 (BioLegend Cat. No. 369611)
AB_2632874 (BioLegend Cat. No. 369612)

Antigen Details

Structure
Ig superfamily and 33 kD.
Distribution

Activated T cells and B cells.

Function
Negative regulator of T cell activation.
Interaction
Antigen presenting cells, such as dendritic cells.
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86).
Cell Type
B cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Kuiper HM, et al. 1995. J. Immunol. 155:1776.
2. Castan J, et al. 1997. Immunology 90:265.
3. Lee CC, et al. 2009. Pediatr. Allergy Immunol. 20:624.
4. Pistillo MP, et al. 2003. Blood 101:202.
5. Tan PH, et al. 2005. Blood. 106:2936.
6. Steiner K, et al. 2001. Clin. Exp. Immunol. 126:143.

Gene ID
1493 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/07/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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