Anti-GPP130 Antibody (Previously Covance catalog# PRB-144C)

Pricing & Availability
Clone
Poly19238 (See other available formats)
Other Names
GIMPC, GOLPH4, Golgi Integral Membrane Protein 4, Golgi Phosphoprotein 4, Type Ii Golgi Membrane Protein, Golgi Phosphoprotein Of 130 kD, Golgi Integral Membrane Protein, Cis, 130 kD Golgi-Localized Phosphoprotein, Cis Golgi-
Previously
Covance Catalog# PRB-144C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly19238_Anti_GPP130_Antibody_012318
Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B-D) GPP130 primary Antibody (Poly19238). Alexa Fluor® 488 (Green) Donkey anti-Rabbit IgG (Cat. No. 406416) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/20, and (B-D) is 1/60. Concentrations for (A, B) is 1/100, (C) is 1/250, (D) is 1/500.
  • Poly19238_Anti_GPP130_Antibody_012318
    Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B-D) GPP130 primary Antibody (Poly19238). Alexa Fluor® 488 (Green) Donkey anti-Rabbit IgG (Cat. No. 406416) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/20, and (B-D) is 1/60. Concentrations for (A, B) is 1/100, (C) is 1/250, (D) is 1/500.
Cat # Size Price Quantity Avail. Save
923801 200 µl 272€
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Description

The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain. GPP130 has been a revealing marker of early Golgi vesicle trafficking.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Monkey, Rabbit, Mouse, Rat, Rodent, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against a recombinant GST-GPP130 fusion protein.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:100 - 1:250 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunoprecipitation (IP).

*Predicted MW = 130 kD


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Puri S, et al. 2002. Traffic. 3:641.
  2. Bachert C, et al. 2001. Mol Biol Cell. 12:3152.
  3. Linstedt AD, et al. 1997. Mol Biol Cell. 8:1073.
Product Citations
  1. Hasegawa H, et al. 2016. Biochim Biophys Acta. 1863: 1534-1551. PubMed
  2. Lee G, et al. 2016. J Cell Biol. 215(5):705-718. PubMed
  3. Choi EK, et al. 2018. Sci Rep. 8:3163. PubMed
  4. Groux-Degroote S, et al. 2018. FEBS J. 285:3442. PubMed
Publication Library
RRID
AB_2565442 (BioLegend Cat. No. 923801)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers, Phospho-Proteins
Gene ID
27333 View all products for this Gene ID
UniProt
View information about GPP130 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/04/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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