Alexa Fluor® 700 anti-mouse Ig light chain κ Antibody

Pricing & Availability
Clone
RMK-45 (See other available formats)
Other Names
Immunoglobulin light chain kappa
Isotype
Rat IgG
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Product Citations
publications
RMK-45_AF700_IgK_Antibody_FC_061714
C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • RMK-45_AF700_IgK_Antibody_FC_061714
    C57BL/6 splenocytes were stained with anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • RMK-45_AF700_IgK_Antibody_FC_2_061714
    Human peripheral blood lymphocytes were stained with (filled histogram) or without (open histogram) purified mouse IgG1, κ ant-human CD3 (clone UCHT1), followed by anti-mouse Ig light chain κ (clone RMK-45) Alexa Fluor® 700.
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
409507 25 µg 84€
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409508 100 µg 220€
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Description

The RMK-45 monoclonal antibody reacts with immunoglobulin light chain kappa in all tested mouse haplotype (Igh-a and b). It does not react with the lambda chain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The RMK-12 monoclonal antibody may be used as a primary antibody for ELISA or as secondary for detection of mouse Ig kappa.

Product Citations
  1. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
Publication Library
RRID
AB_2563582 (BioLegend Cat. No. 409507)
AB_2563583 (BioLegend Cat. No. 409508)

Antigen Details

Structure
Immunoglobulin family
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Protein Synthesis
Antigen References

1. Ludwig TE, et al. 2006. Nat. Methods. 3:637.
2. Nguyen DH, et al. 2005. J. Immunol. 175:228.
3. Bardor M, et al. 2005. J. Biol. Chem. 280:4228.
4. Diaz SL, et al. 2009. PLoS One. 4:e4241.

Gene ID
243469 View all products for this Gene ID
UniProt
View information about Ig light chain kappa on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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