Alexa Fluor® 660 anti-human CD45 Antibody, CD45, HI30

Pricing & Availability

Clone: HI30 (See other available formats)
Regulatory Status: RUO
Workshop: IV N816
Other Names: LCA, T200
Isotype: Mouse IgG1, κ
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Product Citations: publications

HI30_A660_CD45_Antibody_033021 — Human peripheral blood lymphocytes were stained with CD45 (clone HI30) Alexa Fluor® 660 (filled histogram) or unstained cells (open histogram).

Compare all formats See Alexa Fluor® 660 spectral data

Cat #

Size

Price

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304079

25 tests

90€

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304080

100 tests

212€

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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 660 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 660 has an excitation maximum of 663 nm, and a maximum emission of 690 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹, Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Kishihara K, et al. 1993. Cell 74:143.
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
Nagano M, et al. 2007. Blood 110:151.
Jiang Q, et al. 2008. Blood 112:2858. PubMed
Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
Lee J, et al. 2015. J Exp Med. 212:385. PubMed
Breton G, et al. 2015. J Exp Med. 212:401. PubMed
Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2892354 (BioLegend Cat. No. 304079)
AB_2892354 (BioLegend Cat. No. 304080)

Antigen Details

Structure

Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)

Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function

TCR and BCR mediated activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Cell Type

Hematopoietic stem and progenitors, Mesenchymal Stem Cells

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

Thomas M. 1989. Annu. Rev. Immunol. 7:339.
Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID

5788 View all products for this Gene ID

UniProt

View information about CD45 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

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Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/30/2021

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