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Alexa Fluor® 647 anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_A647_CD223_Antibody_051420
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD3ε (clone 145-2C11) Alexa Fluor® 488 and CD223 (LAG-3) (clone C9B7W) Alexa Fluor® 647 (left) or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
  • C9B7W_A647_CD223_Antibody_051420
    ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD3ε (clone 145-2C11) Alexa Fluor® 488 and CD223 (LAG-3) (clone C9B7W) Alexa Fluor® 647 (left) or rat IgG1, κ Alexa Fluor® 647 isotype control (right).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
125241 25 µg 95€
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125242 100 µg 212€
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Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
RRID
AB_2860660 (BioLegend Cat. No. 125241)
AB_2860660 (BioLegend Cat. No. 125242)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/14/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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