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Alexa Fluor® 647 anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
a-C068C2_AF647_1_082911
Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • a-C068C2_AF647_1_082911
    Thioglycollate-elicited BALB/c peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) Alexa Fluor® 647 (top) or rat IgG2a, κ Alexa Fluor® 647 isotype control (bottom).
  • b-C068C2_AF647_2_082911
  • c-C068C2_A647_CD206_Antibody_3_120420
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/mL of CD206 (clone C068C2) Alexa Fluor® 647 (red), 5 µg/mL of CD3 (clone 145‐2C11) Alexa Fluor® 488 (green), and 5 µg/mL of B220 (clone RA3‐6B2) Alexa Fluor® 594 (blue) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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141711 25 µg 104€
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141712 100 µg 235€
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed
  2. Barkaway A, et al. 2021. Immunity. . PubMed
  3. Chandran S, et al. 2020. Front Immunol. 11:787. PubMed
  4. Song Y, et al. 2013. Eur J Pharmacol. 705:49. PubMed
  5. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  6. Kim YS, et al. 2021. Cell Death Dis. 12:307. PubMed
  7. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  8. Tang E, et al. 2015. J Lipid Res. 56:358. PubMed
  9. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  10. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  11. Helsley RN, et al. 2020. eLife. 8:e49882.. PubMed
  12. Howell KL, et al. 2021. FASEB J. 35:e21618. PubMed
  13. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  14. Calvo-Barreiro L, et al. 2021. Neurotherapeutics. . PubMed
  15. Brykczynska U, et al. 2020. Cell Reports. 30(5):1627-1643.e7.. PubMed
  16. Sato H, et al. 2020. Cell Reports. 31(5):107579. PubMed
  17. Xu Z, et al. 2022. Int J Mol Sci. 23:. PubMed
  18. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  19. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  20. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  21. Enam SF, et al. 2020. J Neuroinflammation. 0.845138889. PubMed
  22. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
  23. Lee DY, et al. 2020. Chonnam Med J. 56:12:00. PubMed
  24. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  25. Herrada AA, et al. 2022. Front Immunol. 13:841641. PubMed
  26. De Simone G, et al. 2021. Immunity. :. PubMed
  27. Garre JM, et al. 2020. Nat Commun. 11:1784. PubMed
  28. Li H, et al. 2022. Gut Microbes. 14:2127456. PubMed
  29. Blriot C, et al. 2021. Immunity. :. PubMed
  30. Ren Y, et al. 2020. Stem Cell Res Ther. 0.743055556. PubMed
  31. Wang H, et al. 2016. PLoS One. 11:e0167307. PubMed
  32. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  33. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  34. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  35. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  36. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  37. He S, et al. 2021. Front Pharmacol. 12:743837. PubMed
  38. Zhu Q, et al. 2022. Atherosclerosis. 357:41. PubMed
  39. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  40. Roux C, et al. 2021. Antioxidants (Basel). 10:. PubMed
  41. Gao L, et al. 2021. Exp Ther Med. 22:1367. PubMed
  42. Put S, et al. 2013. J Nucl Med. 54:807. PubMed
  43. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  44. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  45. Budhu S, et al. 2021. Cell Reports. 34(2):108620. PubMed
  46. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  47. Eixarch H, et al. 2020. Neurotherapeutics. 17:1988. PubMed
  48. De Micheli AJ, et al. 2020. Am J Physiol Cell Physiol. 319:C885. PubMed
  49. Li H, et al. 2018. Nat Commun. 9:272. PubMed
  50. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  51. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  52. Chakraborty A, et al. 2021. Biomaterials. 273:120796. PubMed
RRID
AB_10900240 (BioLegend Cat. No. 141711)
AB_10900420 (BioLegend Cat. No. 141712)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 6    Revision Date: 12/04/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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