- MIH18 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- B7-DC, PD-L2, PDL2, B7DC
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. Clone MIH18 is reported to block PDL2.Product Details
- Antibody Type
- Host Species
- Human B7-DC transfected cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking4,5, and immunohistochemistry in frozen sections2 and paraffin-embedded formalin-fixed sections6. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 345504).
(PubMed link indicates BioLegend citation)
- Youngnak-Piboonratankit P, et al. 2004. Immunol. Lett. 94:215. (IHC, IF)
- Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947. (IHC, IF)
- Hobo W, et al. 2010. Blood 25:4501. (FC)
- Nagamatsu T, et al. 2009. Hum. Reprod. 24:3160. (Block)
- Alvarez IB, et al. 2010. J. Infect. Dis. 202:524. (Block)
- Taube JM, et al. 2014. Clin. Cancer Res. 19:5064. (IHC) PubMed
- Product Citations
AB_2728312 (BioLegend Cat. No. 345513)
AB_2728313 (BioLegend Cat. No. 345514)
- B7 Immunoglobulin superfamily, 25 kD
Dendritic cells, liver, few transformed cell lines, subset of macrophages
- Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
- Cell Type
- Dendritic cells, Macrophages
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
2. Ohigashi Y, et al. 2005. Clin. Cancer. Res. 8:2947.
- Gene ID
- 80380 View all products for this Gene ID
- View information about CD273 on UniProt.org
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